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Dreyer,  Susanne Elisabeth

Aqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquids

Rostock : Universität , 2008

https://doi.org/10.18453/rosdok_id00000430

http://purl.uni-rostock.de/rosdok/id00000430

Abstract:

Within the scope of this thesis, the application of ionic liquids (IL) for the generation of aqueous two-phase systems (IL-based ATPS) and their use for the purification of catalytically active enzymes was evaluated. By studying the partitioning of different model proteins, a deeper understanding of the mechanisms influencing the partition process is provided. The IL AmmoengTM 110 in combination with an inorganic salt (K2HPO4/KH2PO4) can be used to establish an IL-based ATPS for the selective purification of two alcohol dehydrogenases. The presence of IL within the system was found to be advantageous due to its stability enhancing effect on both enzymes and its solubility enhancing effect on hydrophobic substrates.

Dissertation Open Access


Einrichtung :
Mathematisch-Naturwissenschaftliche Fakultät
Gutachter :
Kragl,  Udo  (Prof. Dr.)
Bornscheuer,  Uwe  (Prof. Dr.)
Hubbuch,  Jürgen  (Prof. Dr.)
Jahr der Abgabe:
2008
Jahr der Verteidigung:
2009
Sprache(n) :
Englisch
Schlagworte:
purification, model protein, alcohol dehydrogenase, ATPS
DDC Klassifikation :
540 Chemie
URN :
urn:nbn:de:gbv:28-diss2009-0038-8
Persistente URL:
http://purl.uni-rostock.de/rosdok/id00000430
erstellt am:
2009-02-27
zuletzt geändert am:
2018-06-30
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