|The multi-locus sequence typing and the pulsed-field gel electrophoresis were used for analysing the population structure of Bartonella henselae isolates. Through sequencing of the 16S rDNA gene and eight housekeeping genes 14 sequence types (ST) were identified. The comparison of PFGE results with MLST results lead to high concordance between PFGE types and ST´s. Genetic variants, which represent one genetic main type after a lot of passages, were demonstrated for few primary isolates. Our data indicate for the first time, that a few B. h. isolate possess two different 16S rRNA gene copies.