|This study presents a comprehensive RT-PCR system to detect and distinguish between Chlamydophila pneumoniae, Mycoplasma pneumoniae, Legionella pneumophila, Legionella spp. and Mycobacterium tuberculosis complex (MTC) bacteria in respiratory specimens. All reactions take place on one platform. TaqMan probes allow a rapid, simultaneous amplification and distinction. All assays were carefully tested, validated, CE-certificated and are regularly performed in every day routine diagnostics.