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Storm,  Thilo

Influence of polymer surface modification and specific growth factor stimulation on cellular adhesion at the implant/endothelium interface

Rostock : Universität , 2016

https://doi.org/10.18453/rosdok_id00001772

http://purl.uni-rostock.de/rosdok/id00001772

Abstract:

The response of human endothelial cells (ECs) to poly(ε-caprolactone) (PCL) and eight different PCL modifications was examined in vitro. In CLSM, cell proliferation and viability testing, ECs responded best to plasma chemical activation of PCL. When ECs were additionally stimulated by VEGF, their maximum response was again seen on plasma activated PCL. Polymer precoating with matrix proteins was found to be beneficial for EC adhesion. A qPCR study of eNOS, IL-8, ICAM-1, PECAM-1 and VCAM-1 transcripts after VEGF stimulation of ECs on four modified and coated PCL surfaces was also performed.

Dissertation Open Access


Einrichtung :
Mathematisch-Naturwissenschaftliche Fakultät
Gutachter :
Piechulla,  Birgit  (Prof. Dr.)
Hovakimyan,  Marina  (PD Dr.)
Wilhelmi,  Mathias  (Prof. Dr.)
Jahr der Abgabe:
2014
Jahr der Verteidigung:
2015
Sprache(n) :
Englisch
Schlagworte:
coronary artery disease, HUVEC HCAEC, PCL biomaterial functionalization, VEGF, ECM protein precoating, mRNA expression of endothelial marker molecules
DDC Klassifikation :
570 Biowissenschaften, Biologie
URN :
urn:nbn:de:gbv:28-diss2016-0085-0
Persistente URL:
http://purl.uni-rostock.de/rosdok/id00001772
erstellt am:
2016-07-18
zuletzt geändert am:
2018-06-30
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