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Ni,  Xiaoqi

Electrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxide

Rostock : Universität , 2018

https://doi.org/10.18453/rosdok_id00002044

http://purl.uni-rostock.de/rosdok/id00002044

Abstract:

This dissertation describes the hybridization assay on gold electrodes for electrochemical detection of DNA and polymerase chain reaction (PCR) products by means of the electrochemically active compound osmium tetroxide bipyridine. Single base mismatches can be discriminated for their different thermal behavior. We further optimized protective-strand design, surface-probe density, and hybridization temperature. Besides, we also investigated to obtain labeled single stranded DNA targets from asymmetric PCR after labeling forward primer with osmium tetroxide for electrochemical detection.

Dissertation Open Access


Einrichtung :
Mathematisch-Naturwissenschaftliche Fakultät
Gutachter :
Flechsig,  Gerd-Uwe  (Prof. Dr.)
Langer,  Peter  (Prof. Dr.)
Jahr der Abgabe:
2016
Jahr der Verteidigung:
2017
Sprache(n) :
Englisch
Schlagworte:
sandwich hybridization, gold electrode, single base mismatch
DDC Klassifikation :
540 Chemie
URN :
urn:nbn:de:gbv:28-diss2018-0038-9
Persistente URL:
http://purl.uni-rostock.de/rosdok/id00002044
erstellt am:
2018-03-23
zuletzt geändert am:
2018-06-30
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