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Voronina,  Natalia

Production and characterization of genetically modified magnetized cells for future targeted treatment of cardiovascular diseases

Rostock : Universität , 2018

https://doi.org/10.18453/rosdok_id00002056

http://purl.uni-rostock.de/rosdok/id00002056

Abstract:

Gene therapy is a promising tool for cardiovascular disease treatment. Its wide clinical application is, however, limited by the lack of suitable targeted gene delivery systems. In this work, a non-viral vector was tested - composed by polyethyleneimine and magnetic nanoparticles (PEI/MNP) - with primary endothelial cells as in vitro model for angiogenesis and CD133+ hematopoietic stem cells. The obtained results indicate that transient genetic modification of cells with miRNA delivered by PEI/MNP is feasible and results in their magnetization sufficient for targeting and MRI detection.

Dissertation Open Access


Einrichtung :
Universitätsmedizin
Gutachter :
Pfeifer,  Alexander  (Prof. Dr.)
David,  Robert  (Prof. Dr.)
Glocker,  Michael O.  (Prof. Dr.)
Jahr der Abgabe:
2016
Jahr der Verteidigung:
2017
Sprache(n) :
Englisch
Schlagworte:
gene therapy, stem cell engineering, magnetically guided therapy
DDC Klassifikation :
610 Medizin, Gesundheit
URN :
urn:nbn:de:gbv:28-diss2018-0050-4
Persistente URL:
http://purl.uni-rostock.de/rosdok/id00002056
erstellt am:
2018-04-11
zuletzt geändert am:
2018-06-30
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