Einrichtung : |
|
||
---|---|---|---|
Gutachter : |
|
||
Jahr der Abgabe: |
|
||
Jahr der Verteidigung: |
|
Sprache(n) : |
|
|
---|---|---|
Schlagworte: |
|
|
DDC Klassifikation : |
|
URN : |
|
---|
Persistente URL: |
|
|
---|---|---|
erstellt am: |
|
|
zuletzt geändert am: |
|
Volltext |
|
---|
Khani, Afsaneh
Rostock : Universität , 2018
https://doi.org/10.18453/rosdok_id00002093
http://purl.uni-rostock.de/rosdok/id00002093
We investigated gene expression regulation by a putative glycine(Gly) riboswitch located in the 5′-UTR of a SAF protein gene in the S.pyogenes. Gly-dependency was studied using a luciferase reporter gene system. Maximal reporter gene expression happened in the presence of low Gly concentrations. RT-qPCR showed that in the presence of Gly (≥1 mM), expression of the gene was downregulated. Growth in the presence of 0.1 mM Gly led to the production of a full-length transcript. We conclude that the Gly riboswitch in S. pyogenes represses gene expression in the presence of high Gly concentrations.
Dissertation
Open Access
Einrichtung : |
|
||
---|---|---|---|
Gutachter : |
|
||
Jahr der Abgabe: |
|
||
Jahr der Verteidigung: |
|
Sprache(n) : |
|
|
---|---|---|
Schlagworte: |
|
|
DDC Klassifikation : |
|
URN : |
|
---|
Persistente URL: |
|
|
---|---|---|
erstellt am: |
|
|
zuletzt geändert am: |
|
Volltext |
|
---|