10.18453/rosdok_id00000346 Zimmermann, Vera Vera Zimmermann http://d-nb.info/gnd/136003850 Universität Rostock 2008 540 Chemistry & allied sciences 2008 en http://purl.uni-rostock.de/rosdok/id00000346 urn:nbn:de:gbv:28-diss2008-0079-5 A model describing the complete enzyme catalysed synthesis of N acetylneuraminic acid (Neu5Ac is presented. The model is expedient to predict the reaction course for various conditions and therefore to calculate reaction times and yields. Using the model, an optimisation of reaction conditions in consideration of different targets was performed. Furthermore, a new method for the purification of Neu5Ac was developed. For the extraction a two step mechanism was assumed. A kinetic model was developed and combined with the model for reaction to simulate the integration of reaction and extraction.