mycoreobjectrosdok_disshab_0000000182rosdok_disshab_0000000182rosdokdisshabrosdok_derivate_00000037542023-08-08T10:00:41.145Z2009-02-27T14:51:56.140Zadministratorpublished1rosdok_derivate_0000003754truetruederivate_types:fulltextVolltextfulltextwf_edit_epub wf_register_epubstate:publishedstate:publishedveröffentlichtpublishedrosdok/id00000430592712125MODS updated during RosDok migration in June 2021DissertationHochschulschrift137450710Susanne ElisabethDreyer1979-VerfasserInautAqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquidsenProf. Dr.UdoKraglAkademischeR BetreuerIndgsProf. Dr.UweBornscheuerAkademischeR BetreuerIndgsProf. Dr.JürgenHubbuchAkademischeR BetreuerIndgs2147083-2Universität RostockMathematisch-Naturwissenschaftliche FakultätGrad-verleihende Institutiondgg10.18453/rosdok_id00000430http://purl.uni-rostock.de/rosdok/id00000430urn:nbn:de:gbv:28-diss2009-0038-8540 ChemieMathematisch-Naturwissenschaftliche Fakultätfrei zugänglich (Open Access)Lizenz Metadaten: CC0Nutzungsrechte erteiltalle Rechte vorbehaltenUniversität RostockRostock2008monographic20092008Universitätsbibliothek RostockRostock20092009Within the scope of this thesis, the application of ionic liquids (IL) for the generation of aqueous two-phase systems (IL-based ATPS) and their use for the purification of catalytically active enzymes was evaluated. By studying the partitioning of different model proteins, a deeper understanding of the mechanisms influencing the partition process is provided. The IL AmmoengTM 110 in combination with an inorganic salt (K2HPO4/KH2PO4) can be used to establish an IL-based ATPS for the selective purification of two alcohol dehydrogenases. The presence of IL within the system was found to be advantageous due to its stability enhancing effect on both enzymes and its solubility enhancing effect on hydrophobic substrates.purificationmodel proteinalcohol dehydrogenaseATPSUniversitätsbibliothek Rostockhttp://purl.uni-rostock.de/rosdok/id00000430doctype:epubdoctype:epubDokumenttypDocument typedoctype:epub.dissertationdoctype:epub.dissertationDissertationdoctoral thesisdiniPublType:doctoralThesis diniPublType2022:PhDThesis XMetaDissPlusThesisLevel:thesis.doctoralinfo:eu-repo/semantics/doctoralThesisdocumentnatureOfContent:ppn_105825778natureOfContent:ppn_105825778HochschulschriftdiniPublType2022:DoctoralThesisdiniPublType2022:DoctoralThesisDissertation oder HabilitationDoctoral thesisDRIVERdiniPublType2022:PhDThesisdiniPublType2022:PhDThesisDissertationPhD thesisKDSF (Pu34)XMetaDissPlusThesisLevel:thesis.doctoralXMetaDissPlusThesisLevel:thesis.doctoralDoktorarbeitdoctoral thesisrfc5646:enrfc5646:enEnglischEnglishengengSDNB:540SDNB:540540 Chemie540 Chemistry & allied sciencesinstitution:unirostockinstitution:unirostockUniversität RostockUniversity of RostockUniversität RostockUniversität RostockUni.Rostockhttp://d-nb.info/gnd/38329-6institution:unirostock.mnfinstitution:unirostock.mnfMathematisch-Naturwissenschaftliche FakultätFaculty of Mathematics and Natural SciencesUniversität Rostock. Mathematisch-Naturwissenschaftliche FakultätMathematisch-Natur-<br />wissenschaftliche FakultätUni.Rostock.Fakultaet.MNFhttp://d-nb.info/gnd/2147083-2accesscondition:openaccessaccesscondition:openaccessfrei zugänglich (Open Access)open accesshttp://purl.org/coar/access_right/c_abf2OAfreeinfo:eu-repo/semantics/openAccess[DE-28]Open Access$gControlled Vocabulary for Access Rights$uhttp://purl.org/coar/access_right/c_abf2licenseinfo:metadatalicenseinfo:metadataLizenzen für Metadatenlicenseinfo:metadata.cc0licenseinfo:metadata.cc0Lizenz Metadaten: CC0license metadata: CC0/creativecommons/p/zero/1.0/88x31.pnghttps://creativecommons.org/publicdomain/zero/1.0/licenseinfo:depositlicenseinfo:depositVeröffentlichungsgenehmigungpermission to storelicenseinfo:deposit.rightsgrantedlicenseinfo:deposit.rightsgrantedNutzungsrechte erteiltrights grantedlicenseinfo:worklicenseinfo:workWerkworklicenseinfo:work.rightsreservedlicenseinfo:work.rightsreservedalle Rechte vorbehaltenall rights reserved/creativecommons/r/reserved/0.9/88x31.png[DE-28]Urheberrechtsschutz 1.0$gRights Statements$uhttp://rightsstatements.org/vocab/InC/1.0/http://rightsstatements.org/vocab/InC/1.0/http://rightsstatements.org/vocab/InC/1.0/Aqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquidsAqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquidsgnd:137450710gnd:2147083-2gnd:137450710gnd:2147083-2rosdok_disshab_0000000182-d113354e39gnd:137450710Susanne Elisabeth DreyerSusanne Elisabeth Dreyerrosdok_disshab_0000000182-d113354e60Prof. Dr. Udo KraglProf. Dr. Udo Kraglrosdok_disshab_0000000182-d113354e72Prof. Dr. Uwe BornscheuerProf. Dr. Uwe Bornscheuerrosdok_disshab_0000000182-d113354e84Prof. Dr. Jürgen HubbuchProf. Dr. Jürgen Hubbuchrosdok_disshab_0000000182-d113354e97gnd:2147083-2Universität Rostock Mathematisch-Naturwissenschaftliche FakultätUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätSusanne Elisabeth DreyerProf. Dr. Udo KraglProf. Dr. Uwe BornscheuerProf. Dr. Jürgen HubbuchUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätSusanne Elisabeth DreyerProf. Dr. Udo KraglProf. Dr. Uwe BornscheuerProf. Dr. Jürgen HubbuchUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätSusanne Elisabeth DreyerRostockUniversität Rostockepub.dissertation10.18453/rosdok_id00000430http://purl.uni-rostock.de/rosdok/id00000430urn:nbn:de:gbv:28-diss2009-0038-8purificationmodel proteinalcohol dehydrogenaseATPSWithin the scope of this thesis, the application of ionic liquids (IL) for the generation of aqueous two-phase systems (IL-based ATPS) and their use for the purification of catalytically active enzymes was evaluated. By studying the partitioning of different model proteins, a deeper understanding of the mechanisms influencing the partition process is provided. The IL AmmoengTM 110 in combination with an inorganic salt (K2HPO4/KH2PO4) can be used to establish an IL-based ATPS for the selective purification of two alcohol dehydrogenases. The presence of IL within the system was found to be advantageous due to its stability enhancing effect on both enzymes and its solubility enhancing effect on hydrophobic substrates.20082008epub.dissertation1 Dissertation_Dreyer_2009.pdf 2257952 b96dc8dc110bd73b0669a78e20f3919b rosdok/id00000430592712125MODS updated during RosDok migration in June 2021DissertationHochschulschrift137450710Susanne ElisabethDreyer1979-VerfasserInautAqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquidsenProf. Dr.UdoKraglAkademischeR BetreuerIndgsProf. Dr.UweBornscheuerAkademischeR BetreuerIndgsProf. Dr.JürgenHubbuchAkademischeR BetreuerIndgs2147083-2Universität RostockMathematisch-Naturwissenschaftliche FakultätGrad-verleihende Institutiondgg10.18453/rosdok_id00000430http://purl.uni-rostock.de/rosdok/id00000430urn:nbn:de:gbv:28-diss2009-0038-8540 ChemieMathematisch-Naturwissenschaftliche Fakultätfrei zugänglich (Open Access)Lizenz Metadaten: CC0Nutzungsrechte erteiltalle Rechte vorbehaltenUniversität RostockRostock2008monographic20092008Universitätsbibliothek RostockRostock20092009Within the scope of this thesis, the application of ionic liquids (IL) for the generation of aqueous two-phase systems (IL-based ATPS) and their use for the purification of catalytically active enzymes was evaluated. By studying the partitioning of different model proteins, a deeper understanding of the mechanisms influencing the partition process is provided. The IL AmmoengTM 110 in combination with an inorganic salt (K2HPO4/KH2PO4) can be used to establish an IL-based ATPS for the selective purification of two alcohol dehydrogenases. The presence of IL within the system was found to be advantageous due to its stability enhancing effect on both enzymes and its solubility enhancing effect on hydrophobic substrates.purificationmodel proteinalcohol dehydrogenaseATPSUniversitätsbibliothek Rostockhttp://purl.uni-rostock.de/rosdok/id00000430 2009-02-27T14:51:56.140Z 2023-08-08T10:00:41.145Z 2023-08-18T10:00:41.149Z {"identifier":"rosdok/id00000430","type":"local_id","additional":"","service":"MCRLocalID","created":"2018-06-30T12:36:21.957Z"} {"identifier":"http://purl.uni-rostock.de/rosdok/id00000430","type":"purl","additional":"","service":"RosDokPURL","created":"2018-06-30T12:36:22.153Z","registered":"2018-06-30T12:36:22.153Z"} {"identifier":"10.18453/rosdok_id00000430","type":"doi","additional":"","service":"RosDokDOI","created":"2018-06-30T12:36:23.444Z","registered":"2018-06-30T12:36:23.444Z"} {"identifier":"urn:nbn:de:gbv:28-diss2009-0038-8","type":"dnbUrn","additional":"","service":"RosDokURN","created":"2018-06-30T12:36:21.961Z","registered":"2009-02-28T03:24:44.260Z"} administratorfulltextfile/rosdok_disshab_0000000182/rosdok_derivate_0000003754/Dissertation_Dreyer_2009.pdfAqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquidsAqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquidsgnd:137450710gnd:2147083-2gnd:137450710gnd:2147083-2rosdok_disshab_0000000182-d113354e39gnd:137450710Susanne Elisabeth DreyerSusanne Elisabeth Dreyerrosdok_disshab_0000000182-d113354e60Prof. Dr. Udo KraglProf. Dr. Udo Kraglrosdok_disshab_0000000182-d113354e72Prof. Dr. Uwe BornscheuerProf. Dr. Uwe Bornscheuerrosdok_disshab_0000000182-d113354e84Prof. Dr. Jürgen HubbuchProf. Dr. Jürgen Hubbuchrosdok_disshab_0000000182-d113354e97gnd:2147083-2Universität Rostock Mathematisch-Naturwissenschaftliche FakultätUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätSusanne Elisabeth DreyerProf. Dr. Udo KraglProf. Dr. Uwe BornscheuerProf. Dr. Jürgen HubbuchUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätSusanne Elisabeth DreyerProf. Dr. Udo KraglProf. Dr. Uwe BornscheuerProf. Dr. Jürgen HubbuchUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätSusanne Elisabeth DreyerRostockUniversität Rostockepub.dissertation10.18453/rosdok_id00000430http://purl.uni-rostock.de/rosdok/id00000430urn:nbn:de:gbv:28-diss2009-0038-8purificationmodel proteinalcohol dehydrogenaseATPSWithin the scope of this thesis, the application of ionic liquids (IL) for the generation of aqueous two-phase systems (IL-based ATPS) and their use for the purification of catalytically active enzymes was evaluated. By studying the partitioning of different model proteins, a deeper understanding of the mechanisms influencing the partition process is provided. The IL AmmoengTM 110 in combination with an inorganic salt (K2HPO4/KH2PO4) can be used to establish an IL-based ATPS for the selective purification of two alcohol dehydrogenases. The presence of IL within the system was found to be advantageous due to its stability enhancing effect on both enzymes and its solubility enhancing effect on hydrophobic substrates.20082008[xslt]Saxonrosdok/id00000430https://purl.uni-rostock.de/rosdok/id0000043059271212510.18453/rosdok_id00000430urn:nbn:de:gbv:28-diss2009-0038-8Susanne Elisabeth DreyerDreyer Susanne ElisabethAqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquidsDissertationdoctoral thesisUniversität Rostock, 2008Within the scope of this thesis, the application of ionic liquids (IL) for the generation of aqueous two-phase systems (IL-based ATPS) and their use for the purification of catalytically active enzymes was evaluated. By studying the partitioning of different model proteins, a deeper understanding of…Susanne Elisabeth DreyerAqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquidsUniversitätsbibliothek Rostockhttp://purl.uni-rostock.de/rosdok/id00000430137450710Susanne ElisabethDreyer1979-VerfasserInautProf. Dr.UdoKraglAkademischeR BetreuerIndgsProf. Dr.UweBornscheuerAkademischeR BetreuerIndgsProf. Dr.JürgenHubbuchAkademischeR BetreuerIndgs2147083-2Universität RostockMathematisch-Naturwissenschaftliche FakultätGrad-verleihende Institutiondgg[doi]10.18453/rosdok_id00000430[purl]http://purl.uni-rostock.de/rosdok/id00000430[urn]urn:nbn:de:gbv:28-diss2009-0038-8open_access20082008epoch:21th_centuryrfc5646:endoctype:epub.dissertationaccesscondition:openaccessSDNB:540institution:unirostock.mnfstate:published