Molecular mechanisms controlling the tissue-specific activity of the nutritionally regulated promoter I of the acetyl-CoA carboxylase-alpha encoding gene in cattle

10.18453/rosdok_id00000561 Shi, Xuanming Xuanming Shi http://d-nb.info/gnd/13974049X Molecular mechanisms controlling the tissue-specific activity of the nutritionally regulated promoter I of the acetyl-CoA carboxylase-alpha encoding gene in cattle Universität Rostock 2009 570 Life science 2009 en http://purl.uni-rostock.de/rosdok/id00000561 urn:nbn:de:gbv:28-diss2009-0184-9 We analyzed molecular mechanisms controlling the activity of the nutritionally regulated promoter I (PI) of the bovine acetyl-CoA-carboxylase-alpha gene. PI-activity is driven by NF-Y and C/EBP factors having bound to high affinity sites on the proximal PI. However, both factors may also bind to low affinity sites on the distal PI. From there, they act as repressors of PI. We found that NF-Y may diminish and block C/EBP-beta mediated PI activation, even in trans, through direct factor interaction. Alterations in the concentrations of both factors apparently regulate the tissue specific PI-activity.