10.18453/rosdok_id00000571
Wang, Xinchao
Xinchao
Wang
http://d-nb.info/gnd/139910794
Streptamer technology allows to isolate leukemia antigen-specific CD8+ T cells
Universität Rostock
2009
610 Medical sciences Medicine
2009
en
http://purl.uni-rostock.de/rosdok/id00000571
urn:nbn:de:gbv:28-diss2009-0196-0
In this work we investigated whether streptamer technology could purify WT1-specific CD8+ T cells, what is important for the development of adoptive immunotherapy. Sample from HLA/A2+ HDs were identified and selected by streptamer. The function of selected CD8+ T cells was identified by the staining
of phenotypic markers. The results showed that streptamer permits the detection and selection of WT1-specific CD8+ T cells in the PBMCs from HDs. The naïve function of selected CD8+ T cells was preserved and most selected CD8+ T cells demonstrated an effector T cell immunophenotype.