10.18453/rosdok_id00000571 Wang, Xinchao Xinchao Wang http://d-nb.info/gnd/139910794 Universität Rostock 2009 610 Medical sciences Medicine 2009 en http://purl.uni-rostock.de/rosdok/id00000571 urn:nbn:de:gbv:28-diss2009-0196-0 In this work we investigated whether streptamer technology could purify WT1-specific CD8+ T cells, what is important for the development of adoptive immunotherapy. Sample from HLA/A2+ HDs were identified and selected by streptamer. The function of selected CD8+ T cells was identified by the staining of phenotypic markers. The results showed that streptamer permits the detection and selection of WT1-specific CD8+ T cells in the PBMCs from HDs. The naïve function of selected CD8+ T cells was preserved and most selected CD8+ T cells demonstrated an effector T cell immunophenotype.