10.18453/rosdok_id00001119
Lederer, Franziska
Franziska
Lederer
http://d-nb.info/gnd/1032376813
Genetic characterization, heterologous expression and application of S-layer proteins from the bacterial isolates Lysinibacillus sphaericus JG-B53 and Lysinibacillus sphaericus JG-A12
Genetische Charakterisierung, heterologe Expression und die Verwendung von S-Layer Proteinen der bakteriellen Isolate Lysinibacillus sphaericus JG-B53 und Lysinibacillus sphaericus JG-A12
Universität Rostock
2012
570 Life science
2012
en
http://purl.uni-rostock.de/rosdok/id00001119
urn:nbn:de:gbv:28-diss2013-0032-9
The bacterial isolates Lysinibacillus sphaericus JG-A12 and JG-B53 were isolated from a uranium mining waste pile that is contaminated with multiple heavy metal ions. Genome analyses of Bacillus sp. JG-B53 were used to identify bacterial strategies to handle heavy metal polluted environments. The heterologous expression of the silent S-layer like protein gene sllB of L. sphaericus JG-A12 in E. coli induced distinct morphological modifications. Investigations identified SllB, to inhibit bacterial cell division. The filamentous structures were used for the design of polyelectrolyte capsules.