mycoreobjectrosdok_disshab_0000001479rosdok_disshab_0000001479rosdokdisshabrosdok_derivate_00000328222023-08-08T10:06:31.578Z2015-12-11T11:48:33.389ZadministratoreditorDpublished1rosdok_derivate_0000032822truetruederivate_types:fulltextVolltextfulltextwf_edit_epub wf_register_epubstate:publishedstate:publishedveröffentlichtpublishedrosdok/id00001658843887001MODS updated during RosDok migration in June 2021DissertationHochschulschrift1080058109AnneBrosemann1986-VerfasserInautBiochemical analysis of the alpha-terpineol/1,8-cineole cyclization reaction of the cineole synthase of Nicotiana suaveolensBiochemische Analyse der alpha-Terpinol/1,8-Cineol Zyklisierungsreaktion der Cineolsynthase aus Nicotiana suaveolensen131807838Porf. Dr.BirgitPiechullaUniversität Rostock, Institut für Biowissenschaften, Abteilung BiochemieAkademischeR BetreuerIndgsProf. Dr.JörgDegenhardtMartin-Luther-Universität Halle-Wittenberg, Arbeitsgruppe Pharmazeutische BiotechnologieAkademischeR BetreuerIndgs2147083-2Universität RostockMathematisch-Naturwissenschaftliche FakultätGrad-verleihende Institutiondgg10.18453/rosdok_id00001658http://purl.uni-rostock.de/rosdok/id00001658urn:nbn:de:gbv:28-diss2015-0208-8570 Biowissenschaften, BiologieMathematisch-Naturwissenschaftliche Fakultätfrei zugänglich (Open Access)Lizenz Metadaten: CC0Nutzungsrechte erteiltalle Rechte vorbehaltenUniversität RostockRostock2015monographic20152015Universitätsbibliothek RostockRostock2015201519 mutants of the cineole synthase of N. suaveolens were generated by site-directed mutagenesis in order to substitute the amino acids of the cineole synthase with amino acids of the equivalent position of the terpineol synthase of N. langsdorfii. The mutant enzyme F266S carrying a phenylalanine replacement by serine showed an inverted product spectrum compared to the wild type enzyme. Terpineol was the major compound in the product profile of the mutant F266S. It is hypothesized that the synthesis of cineole proceeds independent of terpineol and via α-terpinyl hydronium ion (Wise et al. 2002).terpene synthasescation-pi-interactionnucleophilic attackUniversitätsbibliothek Rostockhttp://purl.uni-rostock.de/rosdok/id00001658doctype:epubdoctype:epubDokumenttypDocument typedoctype:epub.dissertationdoctype:epub.dissertationDissertationdoctoral thesisdiniPublType:doctoralThesis diniPublType2022:PhDThesis XMetaDissPlusThesisLevel:thesis.doctoralinfo:eu-repo/semantics/doctoralThesisdocumentnatureOfContent:ppn_105825778natureOfContent:ppn_105825778HochschulschriftdiniPublType2022:DoctoralThesisdiniPublType2022:DoctoralThesisDissertation oder HabilitationDoctoral thesisDRIVERdiniPublType2022:PhDThesisdiniPublType2022:PhDThesisDissertationPhD thesisKDSF (Pu34)XMetaDissPlusThesisLevel:thesis.doctoralXMetaDissPlusThesisLevel:thesis.doctoralDoktorarbeitdoctoral thesisrfc5646:enrfc5646:enEnglischEnglishengengSDNB:570SDNB:570570 Biowissenschaften, Biologie570 Life scienceinstitution:unirostockinstitution:unirostockUniversität RostockUniversity of RostockUniversität RostockUniversität RostockUni.Rostockhttp://d-nb.info/gnd/38329-6institution:unirostock.mnfinstitution:unirostock.mnfMathematisch-Naturwissenschaftliche FakultätFaculty of Mathematics and Natural SciencesUniversität Rostock. Mathematisch-Naturwissenschaftliche FakultätMathematisch-Natur-<br />wissenschaftliche FakultätUni.Rostock.Fakultaet.MNFhttp://d-nb.info/gnd/2147083-2accesscondition:openaccessaccesscondition:openaccessfrei zugänglich (Open Access)open accesshttp://purl.org/coar/access_right/c_abf2OAfreeinfo:eu-repo/semantics/openAccess[DE-28]Open Access$gControlled Vocabulary for Access Rights$uhttp://purl.org/coar/access_right/c_abf2licenseinfo:metadatalicenseinfo:metadataLizenzen für Metadatenlicenseinfo:metadata.cc0licenseinfo:metadata.cc0Lizenz Metadaten: CC0license metadata: CC0/creativecommons/p/zero/1.0/88x31.pnghttps://creativecommons.org/publicdomain/zero/1.0/licenseinfo:depositlicenseinfo:depositVeröffentlichungsgenehmigungpermission to storelicenseinfo:deposit.rightsgrantedlicenseinfo:deposit.rightsgrantedNutzungsrechte erteiltrights grantedlicenseinfo:worklicenseinfo:workWerkworklicenseinfo:work.rightsreservedlicenseinfo:work.rightsreservedalle Rechte vorbehaltenall rights reserved/creativecommons/r/reserved/0.9/88x31.png[DE-28]Urheberrechtsschutz 1.0$gRights Statements$uhttp://rightsstatements.org/vocab/InC/1.0/http://rightsstatements.org/vocab/InC/1.0/http://rightsstatements.org/vocab/InC/1.0/Biochemical analysis of the alpha-terpineol/1,8-cineole cyclization reaction of the cineole synthase of Nicotiana suaveolensBiochemische Analyse der alpha-Terpinol/1,8-Cineol Zyklisierungsreaktion der Cineolsynthase aus Nicotiana suaveolensBiochemical analysis of the alpha-terpineol/1,8-cineole cyclization reaction of the cineole synthase of Nicotiana suaveolensgnd:1080058109gnd:131807838gnd:2147083-2gnd:1080058109gnd:131807838gnd:2147083-2rosdok_disshab_0000001479-d166797e39gnd:1080058109Anne BrosemannAnne Brosemannrosdok_disshab_0000001479-d166797e62gnd:131807838Porf. Dr. Birgit PiechullaPorf. Dr. Birgit Piechullarosdok_disshab_0000001479-d166797e77Prof. Dr. Jörg DegenhardtProf. Dr. Jörg Degenhardtrosdok_disshab_0000001479-d166797e91gnd:2147083-2Universität Rostock Mathematisch-Naturwissenschaftliche FakultätUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätAnne BrosemannPorf. Dr. Birgit PiechullaProf. Dr. Jörg DegenhardtUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätAnne BrosemannPorf. Dr. Birgit PiechullaProf. Dr. Jörg DegenhardtUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätAnne BrosemannRostockUniversität Rostockepub.dissertation10.18453/rosdok_id00001658http://purl.uni-rostock.de/rosdok/id00001658urn:nbn:de:gbv:28-diss2015-0208-8terpene synthasescation-pi-interactionnucleophilic attack19 mutants of the cineole synthase of N. suaveolens were generated by site-directed mutagenesis in order to substitute the amino acids of the cineole synthase with amino acids of the equivalent position of the terpineol synthase of N. langsdorfii. The mutant enzyme F266S carrying a phenylalanine replacement by serine showed an inverted product spectrum compared to the wild type enzyme. Terpineol was the major compound in the product profile of the mutant F266S. It is hypothesized that the synthesis of cineole proceeds independent of terpineol and via α-terpinyl hydronium ion (Wise et al. 2002).20152015epub.dissertation1 Dissertation_Brosemann_2015.pdf 2359064 5f1d8226f8711b59f6f6e57b06127013 rosdok/id00001658843887001MODS updated during RosDok migration in June 2021DissertationHochschulschrift1080058109AnneBrosemann1986-VerfasserInautBiochemical analysis of the alpha-terpineol/1,8-cineole cyclization reaction of the cineole synthase of Nicotiana suaveolensBiochemische Analyse der alpha-Terpinol/1,8-Cineol Zyklisierungsreaktion der Cineolsynthase aus Nicotiana suaveolensen131807838Porf. Dr.BirgitPiechullaUniversität Rostock, Institut für Biowissenschaften, Abteilung BiochemieAkademischeR BetreuerIndgsProf. Dr.JörgDegenhardtMartin-Luther-Universität Halle-Wittenberg, Arbeitsgruppe Pharmazeutische BiotechnologieAkademischeR BetreuerIndgs2147083-2Universität RostockMathematisch-Naturwissenschaftliche FakultätGrad-verleihende Institutiondgg10.18453/rosdok_id00001658http://purl.uni-rostock.de/rosdok/id00001658urn:nbn:de:gbv:28-diss2015-0208-8570 Biowissenschaften, BiologieMathematisch-Naturwissenschaftliche Fakultätfrei zugänglich (Open Access)Lizenz Metadaten: CC0Nutzungsrechte erteiltalle Rechte vorbehaltenUniversität RostockRostock2015monographic20152015Universitätsbibliothek RostockRostock2015201519 mutants of the cineole synthase of N. suaveolens were generated by site-directed mutagenesis in order to substitute the amino acids of the cineole synthase with amino acids of the equivalent position of the terpineol synthase of N. langsdorfii. The mutant enzyme F266S carrying a phenylalanine replacement by serine showed an inverted product spectrum compared to the wild type enzyme. Terpineol was the major compound in the product profile of the mutant F266S. It is hypothesized that the synthesis of cineole proceeds independent of terpineol and via α-terpinyl hydronium ion (Wise et al. 2002).terpene synthasescation-pi-interactionnucleophilic attackUniversitätsbibliothek Rostockhttp://purl.uni-rostock.de/rosdok/id00001658 2015-12-11T11:48:33.389Z 2023-08-08T10:06:31.578Z 2023-08-18T10:06:31.583Z editorD {"identifier":"rosdok/id00001658","type":"local_id","additional":"","service":"MCRLocalID","created":"2018-06-30T13:20:22.183Z"} {"identifier":"http://purl.uni-rostock.de/rosdok/id00001658","type":"purl","additional":"","service":"RosDokPURL","created":"2018-06-30T13:20:22.363Z","registered":"2018-06-30T13:20:22.363Z"} {"identifier":"10.18453/rosdok_id00001658","type":"doi","additional":"","service":"RosDokDOI","created":"2018-06-30T13:20:23.716Z","registered":"2018-06-30T13:20:23.716Z"} {"identifier":"urn:nbn:de:gbv:28-diss2015-0208-8","type":"dnbUrn","additional":"","service":"RosDokURN","created":"2018-06-30T13:20:22.189Z","registered":"2015-12-15T03:19:34.893Z"} administratorfulltextfile/rosdok_disshab_0000001479/rosdok_derivate_0000032822/Dissertation_Brosemann_2015.pdfBiochemical analysis of the alpha-terpineol/1,8-cineole cyclization reaction of the cineole synthase of Nicotiana suaveolensBiochemische Analyse der alpha-Terpinol/1,8-Cineol Zyklisierungsreaktion der Cineolsynthase aus Nicotiana suaveolensBiochemical analysis of the alpha-terpineol/1,8-cineole cyclization reaction of the cineole synthase of Nicotiana suaveolensgnd:1080058109gnd:131807838gnd:2147083-2gnd:1080058109gnd:131807838gnd:2147083-2rosdok_disshab_0000001479-d166797e39gnd:1080058109Anne BrosemannAnne Brosemannrosdok_disshab_0000001479-d166797e62gnd:131807838Porf. Dr. Birgit PiechullaPorf. Dr. Birgit Piechullarosdok_disshab_0000001479-d166797e77Prof. Dr. Jörg DegenhardtProf. Dr. Jörg Degenhardtrosdok_disshab_0000001479-d166797e91gnd:2147083-2Universität Rostock Mathematisch-Naturwissenschaftliche FakultätUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätAnne BrosemannPorf. Dr. Birgit PiechullaProf. Dr. Jörg DegenhardtUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätAnne BrosemannPorf. Dr. Birgit PiechullaProf. Dr. Jörg DegenhardtUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätAnne BrosemannRostockUniversität Rostockepub.dissertation10.18453/rosdok_id00001658http://purl.uni-rostock.de/rosdok/id00001658urn:nbn:de:gbv:28-diss2015-0208-8terpene synthasescation-pi-interactionnucleophilic attack19 mutants of the cineole synthase of N. suaveolens were generated by site-directed mutagenesis in order to substitute the amino acids of the cineole synthase with amino acids of the equivalent position of the terpineol synthase of N. langsdorfii. The mutant enzyme F266S carrying a phenylalanine replacement by serine showed an inverted product spectrum compared to the wild type enzyme. Terpineol was the major compound in the product profile of the mutant F266S. It is hypothesized that the synthesis of cineole proceeds independent of terpineol and via α-terpinyl hydronium ion (Wise et al. 2002).20152015[xslt]Saxonrosdok/id00001658https://purl.uni-rostock.de/rosdok/id0000165884388700110.18453/rosdok_id00001658urn:nbn:de:gbv:28-diss2015-0208-8Anne BrosemannBrosemann AnneBiochemical analysis of the alpha-terpineol/1,8-cineole cyclization reaction of the cineole synthase of Nicotiana suaveolensDissertationdoctoral thesisUniversität Rostock, 201519 mutants of the cineole synthase of N. suaveolens were generated by site-directed mutagenesis in order to substitute the amino acids of the cineole synthase with amino acids of the equivalent position of the terpineol synthase of N. langsdorfii. The mutant enzyme F266S carrying a phenylalanine…Anne BrosemannBiochemical analysis of the alpha-terpineol/1,8-cineole cyclization reaction of the cineole synthase of Nicotiana suaveolensBiochemische Analyse der alpha-Terpinol/1,8-Cineol Zyklisierungsreaktion der Cineolsynthase aus Nicotiana suaveolensUniversitätsbibliothek Rostockhttp://purl.uni-rostock.de/rosdok/id000016581080058109AnneBrosemann1986-VerfasserInaut131807838Porf. Dr.BirgitPiechullaUniversität Rostock, Institut für Biowissenschaften, Abteilung BiochemieAkademischeR BetreuerIndgsProf. Dr.JörgDegenhardtMartin-Luther-Universität Halle-Wittenberg, Arbeitsgruppe Pharmazeutische BiotechnologieAkademischeR BetreuerIndgs2147083-2Universität RostockMathematisch-Naturwissenschaftliche FakultätGrad-verleihende Institutiondgg[doi]10.18453/rosdok_id00001658[purl]http://purl.uni-rostock.de/rosdok/id00001658[urn]urn:nbn:de:gbv:28-diss2015-0208-8open_access20152015epoch:21th_centuryrfc5646:endoctype:epub.dissertationaccesscondition:openaccessSDNB:570institution:unirostock.mnfstate:published