mycoreobjectrosdok_disshab_0000001505rosdok_disshab_0000001505rosdokdisshabrosdok_derivate_00000328602023-08-08T10:06:39.983Z2015-12-22T09:26:50.210ZadministratoreditorDpublished1rosdok_derivate_0000032860truetruederivate_types:fulltextVolltextfulltextwf_edit_epub wf_register_epubstate:publishedstate:publishedveröffentlichtpublishedrosdok/id00001684856304654MODS updated during RosDok migration in June 2021DissertationHochschulschrift108177682XAnnaSchade1987-VerfasserInautMicroRNA-based stem cell modifications using magnetic transfection complexesA non-viral approach towards cardiac regenerationMikroRNA-basierte Stammzellmodifikationen mittels magnetischer TransfektionskomplexeEin nicht-viraler Ansatz für die kardiale Regenerationen136129560Prof. Dr. med.GustavSteinhoffUniversitätsmedizin Rostock, Klinik und Poliklinik für HerzchirurgieAkademischeR BetreuerIndgs122722388Prof. Dr. med. Dr. rer. nat.Brigitte M.PützerUniversitätsmedizin Rostock, Institut für Experimentelle Gentherapie und TumorforschungAkademischeR BetreuerIndgsPD Dr. med.StefanBrunnerUniversität München, Medizinische Klinik und Poliklinik IAkademischeR BetreuerIndgs1029510660Universität RostockUniversitätsmedizinGrad-verleihende Institutiondgg10.18453/rosdok_id00001684http://purl.uni-rostock.de/rosdok/id00001684urn:nbn:de:gbv:28-diss2015-0234-1610 Medizin, GesundheitUniversitätsmedizinfrei zugänglich (Open Access)Lizenz Metadaten: CC0Nutzungsrechte erteiltalle Rechte vorbehaltenUniversität RostockRostock2015monographic20152015Universitätsbibliothek RostockRostock20152015Regenerative properties of human mesenchymal stem cells (hMSC) can be enhanced by genetic modifications. Thus we aimed to develop a magnetic non-viral vector for microRNA (miR) delivery in freshly isolated hMSCs. miR/PEI/MNP transfection showed a better long term performance compared to miR/PEI transfection. Moreover magnetically modified hMSCs could be guided by an external magnet contributing to enhanced cell engraftment. Conclusively a magnetic non-viral carrier for efficient miR delivery in freshly isolated hMSCs was developed allowing specific control of stem cells and magnetic targeting.magnetic non-viral carriermicroRNAmesenchymal stem cellsmagnetic nanoparticlesmagnetofectionUniversitätsbibliothek Rostockhttp://purl.uni-rostock.de/rosdok/id00001684doctype:epubdoctype:epubDokumenttypDocument typedoctype:epub.dissertationdoctype:epub.dissertationDissertationdoctoral thesisdiniPublType:doctoralThesis diniPublType2022:PhDThesis XMetaDissPlusThesisLevel:thesis.doctoralinfo:eu-repo/semantics/doctoralThesisdocumentnatureOfContent:ppn_105825778natureOfContent:ppn_105825778HochschulschriftdiniPublType2022:DoctoralThesisdiniPublType2022:DoctoralThesisDissertation oder HabilitationDoctoral thesisDRIVERdiniPublType2022:PhDThesisdiniPublType2022:PhDThesisDissertationPhD thesisKDSF (Pu34)XMetaDissPlusThesisLevel:thesis.doctoralXMetaDissPlusThesisLevel:thesis.doctoralDoktorarbeitdoctoral thesisrfc5646:enrfc5646:enEnglischEnglishengengSDNB:610SDNB:610610 Medizin, Gesundheit610 Medical sciences Medicineinstitution:unirostockinstitution:unirostockUniversität RostockUniversity of RostockUniversität RostockUniversität RostockUni.Rostockhttp://d-nb.info/gnd/38329-6institution:unirostock.umrinstitution:unirostock.umrUniversitätsmedizinUniversity MedicineUniversität Rostock. UniversitätsmedizinUniversitätsmedizinUni.Rostock.Fakultaet.MEDhttp://d-nb.info/gnd/1029510660accesscondition:openaccessaccesscondition:openaccessfrei zugänglich (Open Access)open accesshttp://purl.org/coar/access_right/c_abf2OAfreeinfo:eu-repo/semantics/openAccess[DE-28]Open Access$gControlled Vocabulary for Access Rights$uhttp://purl.org/coar/access_right/c_abf2licenseinfo:metadatalicenseinfo:metadataLizenzen für Metadatenlicenseinfo:metadata.cc0licenseinfo:metadata.cc0Lizenz Metadaten: CC0license metadata: CC0/creativecommons/p/zero/1.0/88x31.pnghttps://creativecommons.org/publicdomain/zero/1.0/licenseinfo:depositlicenseinfo:depositVeröffentlichungsgenehmigungpermission to storelicenseinfo:deposit.rightsgrantedlicenseinfo:deposit.rightsgrantedNutzungsrechte erteiltrights grantedlicenseinfo:worklicenseinfo:workWerkworklicenseinfo:work.rightsreservedlicenseinfo:work.rightsreservedalle Rechte vorbehaltenall rights reserved/creativecommons/r/reserved/0.9/88x31.png[DE-28]Urheberrechtsschutz 1.0$gRights Statements$uhttp://rightsstatements.org/vocab/InC/1.0/http://rightsstatements.org/vocab/InC/1.0/http://rightsstatements.org/vocab/InC/1.0/MicroRNA-based stem cell modifications using magnetic transfection complexesA non-viral approach towards cardiac regenerationMikroRNA-basierte Stammzellmodifikationen mittels magnetischer TransfektionskomplexeEin nicht-viraler Ansatz für die kardiale RegenerationMicroRNA-based stem cell modifications using magnetic transfection complexesA non-viral approach towards cardiac regenerationgnd:108177682Xgnd:136129560gnd:122722388gnd:1029510660gnd:108177682Xgnd:136129560gnd:122722388gnd:1029510660rosdok_disshab_0000001505-d4041734e39gnd:108177682XAnna SchadeAnna Schaderosdok_disshab_0000001505-d4041734e64gnd:136129560Prof. Dr. med. Gustav SteinhoffProf. Dr. med. Gustav Steinhoffrosdok_disshab_0000001505-d4041734e79gnd:122722388Prof. Dr. med. Dr. rer. nat. Brigitte M. PützerProf. Dr. med. Dr. rer. nat. Brigitte M. Pützerrosdok_disshab_0000001505-d4041734e95PD Dr. med. Stefan BrunnerPD Dr. med. Stefan Brunnerrosdok_disshab_0000001505-d4041734e108gnd:1029510660Universität Rostock UniversitätsmedizinUniversität Rostock UniversitätsmedizinAnna SchadeProf. Dr. med. Gustav SteinhoffProf. Dr. med. Dr. rer. nat. Brigitte M. PützerPD Dr. med. Stefan BrunnerUniversität Rostock UniversitätsmedizinAnna SchadeProf. Dr. med. Gustav SteinhoffProf. Dr. med. Dr. rer. nat. Brigitte M. PützerPD Dr. med. Stefan BrunnerUniversität Rostock UniversitätsmedizinAnna SchadeRostockUniversität Rostockepub.dissertation10.18453/rosdok_id00001684http://purl.uni-rostock.de/rosdok/id00001684urn:nbn:de:gbv:28-diss2015-0234-1magnetic non-viral carriermicroRNAmesenchymal stem cellsmagnetic nanoparticlesmagnetofectionRegenerative properties of human mesenchymal stem cells (hMSC) can be enhanced by genetic modifications. Thus we aimed to develop a magnetic non-viral vector for microRNA (miR) delivery in freshly isolated hMSCs. miR/PEI/MNP transfection showed a better long term performance compared to miR/PEI transfection. Moreover magnetically modified hMSCs could be guided by an external magnet contributing to enhanced cell engraftment. Conclusively a magnetic non-viral carrier for efficient miR delivery in freshly isolated hMSCs was developed allowing specific control of stem cells and magnetic targeting.20152015epub.dissertation1 Dissertation_Schade_2015.pdf 4867713 570b1d0dfd318ccd7400470bb3b27426 rosdok/id00001684856304654MODS updated during RosDok migration in June 2021DissertationHochschulschrift108177682XAnnaSchade1987-VerfasserInautMicroRNA-based stem cell modifications using magnetic transfection complexesA non-viral approach towards cardiac regenerationMikroRNA-basierte Stammzellmodifikationen mittels magnetischer TransfektionskomplexeEin nicht-viraler Ansatz für die kardiale Regenerationen136129560Prof. Dr. med.GustavSteinhoffUniversitätsmedizin Rostock, Klinik und Poliklinik für HerzchirurgieAkademischeR BetreuerIndgs122722388Prof. Dr. med. Dr. rer. nat.Brigitte M.PützerUniversitätsmedizin Rostock, Institut für Experimentelle Gentherapie und TumorforschungAkademischeR BetreuerIndgsPD Dr. med.StefanBrunnerUniversität München, Medizinische Klinik und Poliklinik IAkademischeR BetreuerIndgs1029510660Universität RostockUniversitätsmedizinGrad-verleihende Institutiondgg10.18453/rosdok_id00001684http://purl.uni-rostock.de/rosdok/id00001684urn:nbn:de:gbv:28-diss2015-0234-1610 Medizin, GesundheitUniversitätsmedizinfrei zugänglich (Open Access)Lizenz Metadaten: CC0Nutzungsrechte erteiltalle Rechte vorbehaltenUniversität RostockRostock2015monographic20152015Universitätsbibliothek RostockRostock20152015Regenerative properties of human mesenchymal stem cells (hMSC) can be enhanced by genetic modifications. Thus we aimed to develop a magnetic non-viral vector for microRNA (miR) delivery in freshly isolated hMSCs. miR/PEI/MNP transfection showed a better long term performance compared to miR/PEI transfection. Moreover magnetically modified hMSCs could be guided by an external magnet contributing to enhanced cell engraftment. 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Gustav SteinhoffProf. Dr. med. Gustav Steinhoffrosdok_disshab_0000001505-d4041734e79gnd:122722388Prof. Dr. med. Dr. rer. nat. Brigitte M. PützerProf. Dr. med. Dr. rer. nat. Brigitte M. Pützerrosdok_disshab_0000001505-d4041734e95PD Dr. med. Stefan BrunnerPD Dr. med. Stefan Brunnerrosdok_disshab_0000001505-d4041734e108gnd:1029510660Universität Rostock UniversitätsmedizinUniversität Rostock UniversitätsmedizinAnna SchadeProf. Dr. med. Gustav SteinhoffProf. Dr. med. Dr. rer. nat. Brigitte M. PützerPD Dr. med. Stefan BrunnerUniversität Rostock UniversitätsmedizinAnna SchadeProf. Dr. med. Gustav SteinhoffProf. Dr. med. Dr. rer. nat. Brigitte M. PützerPD Dr. med. Stefan BrunnerUniversität Rostock UniversitätsmedizinAnna SchadeRostockUniversität Rostockepub.dissertation10.18453/rosdok_id00001684http://purl.uni-rostock.de/rosdok/id00001684urn:nbn:de:gbv:28-diss2015-0234-1magnetic non-viral carriermicroRNAmesenchymal stem cellsmagnetic nanoparticlesmagnetofectionRegenerative properties of human mesenchymal stem cells (hMSC) can be enhanced by genetic modifications. Thus we aimed to develop a magnetic non-viral vector for microRNA (miR) delivery in freshly isolated hMSCs. miR/PEI/MNP transfection showed a better long term performance compared to miR/PEI transfection. Moreover magnetically modified hMSCs could be guided by an external magnet contributing to enhanced cell engraftment. Conclusively a magnetic non-viral carrier for efficient miR delivery in freshly isolated hMSCs was developed allowing specific control of stem cells and magnetic targeting.20152015[xslt]Saxonrosdok/id00001684https://purl.uni-rostock.de/rosdok/id0000168485630465410.18453/rosdok_id00001684urn:nbn:de:gbv:28-diss2015-0234-1Anna SchadeSchade AnnaMicroRNA-based stem cell modifications using magnetic transfection complexes : A non-viral approach towards cardiac regenerationDissertationdoctoral thesisUniversität Rostock, 2015Regenerative properties of human mesenchymal stem cells (hMSC) can be enhanced by genetic modifications. Thus we aimed to develop a magnetic non-viral vector for microRNA (miR) delivery in freshly isolated hMSCs. miR/PEI/MNP transfection showed a better long term performance compared to miR/PEI…Anna SchadeMicroRNA-based stem cell modifications using magnetic transfection complexesA non-viral approach towards cardiac regenerationMikroRNA-basierte Stammzellmodifikationen mittels magnetischer TransfektionskomplexeEin nicht-viraler Ansatz für die kardiale RegenerationUniversitätsbibliothek Rostockhttp://purl.uni-rostock.de/rosdok/id00001684108177682XAnnaSchade1987-VerfasserInaut136129560Prof. Dr. med.GustavSteinhoffUniversitätsmedizin Rostock, Klinik und Poliklinik für HerzchirurgieAkademischeR BetreuerIndgs122722388Prof. Dr. med. Dr. rer. nat.Brigitte M.PützerUniversitätsmedizin Rostock, Institut für Experimentelle Gentherapie und TumorforschungAkademischeR BetreuerIndgsPD Dr. med.StefanBrunnerUniversität München, Medizinische Klinik und Poliklinik IAkademischeR BetreuerIndgs1029510660Universität RostockUniversitätsmedizinGrad-verleihende Institutiondgg[doi]10.18453/rosdok_id00001684[purl]http://purl.uni-rostock.de/rosdok/id00001684[urn]urn:nbn:de:gbv:28-diss2015-0234-1open_access20152015epoch:21th_centuryrfc5646:endoctype:epub.dissertationaccesscondition:openaccessSDNB:610institution:unirostock.umrstate:published