mycoreobjectrosdok_disshab_0000001696rosdok_disshab_0000001696rosdokdisshabrosdok_derivate_00000368062023-08-08T10:07:44.945Z2017-03-27T08:08:39.931ZadministratoreditorDpublished1rosdok_derivate_0000036806truetruederivate_types:fulltextVolltextfulltextwf_edit_epub wf_register_epubstate:publishedstate:publishedveröffentlichtpublishedrosdok/id00001874882994220MODS updated during RosDok migration in June 2021DissertationHochschulschrift1128603144EvgenyaDelyagina1986-VerfasserInautMagnetic nanoparticle-based gene deliverymechanisms and clinical perspectivesMagnetische Nanopartikel-basierter GentransferMechanismen und Klinische Perspektivenen136129560Prof. Dr. med.GustavSteinhoffUniversitätsmedizin Rostock, Klinik und Poliklinik für HerzchirurgieAkademischeR BetreuerIndgs122722388Prof. Dr. med. Dr. rer. nat.Brigitte M.PützerUniversitätsmedizin Rostock, Institut für Experimentelle Gentherapie und TumorforschungAkademischeR BetreuerIndgs1082299979Prof. Dr. rer. nat.MartinZenkeRWTH Aachen, Universitätsklinikum der RWTH, Helmholtz-Institut für Biomedizinische TechnikAkademischeR BetreuerIndgs1029510660Universität RostockUniversitätsmedizinGrad-verleihende Institutiondgg10.18453/rosdok_id00001874http://purl.uni-rostock.de/rosdok/id00001874urn:nbn:de:gbv:28-diss2017-0038-4610 Medizin, GesundheitUniversitätsmedizinfrei zugänglich (Open Access)Lizenz Metadaten: CC0Nutzungsrechte erteiltalle Rechte vorbehaltenUniversität RostockRostock2017monographic20172017Universitätsbibliothek RostockRostock20172017The development of safe and efficient gene delivery systems is the key to the overall success of gene therapy. This work introduced a MNP-based nonviral gene delivery approach for transfection in clinically relevant cells. Moreover, the in vitro mechanism studies could explained the beneficial properties of the proposed carrier design. This work demonstrated, that proposed system could efficiently and safely transfect human bone marrow derived mesenchymal stem cells. Additionaly, the clinical perspectives of MNP-based gene therapy were discussed and evaluated in this dissertation.nonviral transfectionmagnetic nanoparticleshuman mesenchymal stem cellspolyethylenimineUniversitätsbibliothek Rostockhttp://purl.uni-rostock.de/rosdok/id00001874doctype:epubdoctype:epubDokumenttypDocument typedoctype:epub.dissertationdoctype:epub.dissertationDissertationdoctoral thesisdiniPublType:doctoralThesis diniPublType2022:PhDThesis XMetaDissPlusThesisLevel:thesis.doctoralinfo:eu-repo/semantics/doctoralThesisdocumentnatureOfContent:ppn_105825778natureOfContent:ppn_105825778HochschulschriftdiniPublType2022:DoctoralThesisdiniPublType2022:DoctoralThesisDissertation oder HabilitationDoctoral thesisDRIVERdiniPublType2022:PhDThesisdiniPublType2022:PhDThesisDissertationPhD thesisKDSF (Pu34)XMetaDissPlusThesisLevel:thesis.doctoralXMetaDissPlusThesisLevel:thesis.doctoralDoktorarbeitdoctoral thesisrfc5646:enrfc5646:enEnglischEnglishengengSDNB:610SDNB:610610 Medizin, Gesundheit610 Medical sciences Medicineinstitution:unirostockinstitution:unirostockUniversität RostockUniversity of RostockUniversität RostockUniversität RostockUni.Rostockhttp://d-nb.info/gnd/38329-6institution:unirostock.umrinstitution:unirostock.umrUniversitätsmedizinUniversity MedicineUniversität Rostock. UniversitätsmedizinUniversitätsmedizinUni.Rostock.Fakultaet.MEDhttp://d-nb.info/gnd/1029510660accesscondition:openaccessaccesscondition:openaccessfrei zugänglich (Open Access)open accesshttp://purl.org/coar/access_right/c_abf2OAfreeinfo:eu-repo/semantics/openAccess[DE-28]Open Access$gControlled Vocabulary for Access Rights$uhttp://purl.org/coar/access_right/c_abf2licenseinfo:metadatalicenseinfo:metadataLizenzen für Metadatenlicenseinfo:metadata.cc0licenseinfo:metadata.cc0Lizenz Metadaten: CC0license metadata: CC0/creativecommons/p/zero/1.0/88x31.pnghttps://creativecommons.org/publicdomain/zero/1.0/licenseinfo:depositlicenseinfo:depositVeröffentlichungsgenehmigungpermission to storelicenseinfo:deposit.rightsgrantedlicenseinfo:deposit.rightsgrantedNutzungsrechte erteiltrights grantedlicenseinfo:worklicenseinfo:workWerkworklicenseinfo:work.rightsreservedlicenseinfo:work.rightsreservedalle Rechte vorbehaltenall rights reserved/creativecommons/r/reserved/0.9/88x31.png[DE-28]Urheberrechtsschutz 1.0$gRights Statements$uhttp://rightsstatements.org/vocab/InC/1.0/http://rightsstatements.org/vocab/InC/1.0/http://rightsstatements.org/vocab/InC/1.0/Magnetic nanoparticle-based gene deliverymechanisms and clinical perspectivesMagnetische Nanopartikel-basierter GentransferMechanismen und Klinische PerspektivenMagnetic nanoparticle-based gene deliverymechanisms and clinical perspectivesgnd:1128603144gnd:136129560gnd:122722388gnd:1082299979gnd:1029510660gnd:1128603144gnd:136129560gnd:122722388gnd:1082299979gnd:1029510660rosdok_disshab_0000001696-d3863550e39gnd:1128603144Evgenya DelyaginaEvgenya Delyaginarosdok_disshab_0000001696-d3863550e64gnd:136129560Prof. Dr. med. 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Martin ZenkeUniversität Rostock UniversitätsmedizinEvgenya DelyaginaRostockUniversität Rostockepub.dissertation10.18453/rosdok_id00001874http://purl.uni-rostock.de/rosdok/id00001874urn:nbn:de:gbv:28-diss2017-0038-4nonviral transfectionmagnetic nanoparticleshuman mesenchymal stem cellspolyethylenimineThe development of safe and efficient gene delivery systems is the key to the overall success of gene therapy. This work introduced a MNP-based nonviral gene delivery approach for transfection in clinically relevant cells. Moreover, the in vitro mechanism studies could explained the beneficial properties of the proposed carrier design. This work demonstrated, that proposed system could efficiently and safely transfect human bone marrow derived mesenchymal stem cells. 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Dr. rer. nat.Brigitte M.PützerUniversitätsmedizin Rostock, Institut für Experimentelle Gentherapie und TumorforschungAkademischeR BetreuerIndgs1082299979Prof. Dr. rer. nat.MartinZenkeRWTH Aachen, Universitätsklinikum der RWTH, Helmholtz-Institut für Biomedizinische TechnikAkademischeR BetreuerIndgs1029510660Universität RostockUniversitätsmedizinGrad-verleihende Institutiondgg10.18453/rosdok_id00001874http://purl.uni-rostock.de/rosdok/id00001874urn:nbn:de:gbv:28-diss2017-0038-4610 Medizin, GesundheitUniversitätsmedizinfrei zugänglich (Open Access)Lizenz Metadaten: CC0Nutzungsrechte erteiltalle Rechte vorbehaltenUniversität RostockRostock2017monographic20172017Universitätsbibliothek RostockRostock20172017The development of safe and efficient gene delivery systems is the key to the overall success of gene therapy. This work introduced a MNP-based nonviral gene delivery approach for transfection in clinically relevant cells. Moreover, the in vitro mechanism studies could explained the beneficial properties of the proposed carrier design. This work demonstrated, that proposed system could efficiently and safely transfect human bone marrow derived mesenchymal stem cells. Additionaly, the clinical perspectives of MNP-based gene therapy were discussed and evaluated in this dissertation.nonviral transfectionmagnetic nanoparticleshuman mesenchymal stem cellspolyethylenimineUniversitätsbibliothek Rostockhttp://purl.uni-rostock.de/rosdok/id00001874 2017-03-27T08:08:39.931Z 2023-08-08T10:07:44.945Z 2023-08-18T10:07:44.950Z editorD {"identifier":"rosdok/id00001874","type":"local_id","additional":"","service":"MCRLocalID","created":"2018-06-30T13:28:11.645Z"} {"identifier":"http://purl.uni-rostock.de/rosdok/id00001874","type":"purl","additional":"","service":"RosDokPURL","created":"2018-06-30T13:28:11.786Z","registered":"2018-06-30T13:28:11.786Z"} {"identifier":"10.18453/rosdok_id00001874","type":"doi","additional":"","service":"RosDokDOI","created":"2018-06-30T13:28:13.144Z","registered":"2018-06-30T13:28:13.144Z"} {"identifier":"urn:nbn:de:gbv:28-diss2017-0038-4","type":"dnbUrn","additional":"","service":"RosDokURN","created":"2018-06-30T13:28:11.652Z","registered":"2017-03-28T02:28:21.510Z"} administratorfulltextfile/rosdok_disshab_0000001696/rosdok_derivate_0000036806/Dissertation_Delyagina_2017.pdfMagnetic nanoparticle-based gene deliverymechanisms and clinical perspectivesMagnetische Nanopartikel-basierter GentransferMechanismen und Klinische PerspektivenMagnetic nanoparticle-based gene deliverymechanisms and clinical perspectivesgnd:1128603144gnd:136129560gnd:122722388gnd:1082299979gnd:1029510660gnd:1128603144gnd:136129560gnd:122722388gnd:1082299979gnd:1029510660rosdok_disshab_0000001696-d3863550e39gnd:1128603144Evgenya DelyaginaEvgenya Delyaginarosdok_disshab_0000001696-d3863550e64gnd:136129560Prof. Dr. med. Gustav SteinhoffProf. Dr. med. Gustav Steinhoffrosdok_disshab_0000001696-d3863550e79gnd:122722388Prof. Dr. med. Dr. rer. nat. Brigitte M. PützerProf. Dr. med. Dr. rer. nat. Brigitte M. Pützerrosdok_disshab_0000001696-d3863550e95gnd:1082299979Prof. Dr. rer. nat. Martin ZenkeProf. Dr. rer. nat. Martin Zenkerosdok_disshab_0000001696-d3863550e110gnd:1029510660Universität Rostock UniversitätsmedizinUniversität Rostock UniversitätsmedizinEvgenya DelyaginaProf. Dr. med. Gustav SteinhoffProf. Dr. med. Dr. rer. nat. Brigitte M. PützerProf. Dr. rer. nat. Martin ZenkeUniversität Rostock UniversitätsmedizinEvgenya DelyaginaProf. Dr. med. Gustav SteinhoffProf. Dr. med. Dr. rer. nat. Brigitte M. PützerProf. Dr. rer. nat. Martin ZenkeUniversität Rostock UniversitätsmedizinEvgenya DelyaginaRostockUniversität Rostockepub.dissertation10.18453/rosdok_id00001874http://purl.uni-rostock.de/rosdok/id00001874urn:nbn:de:gbv:28-diss2017-0038-4nonviral transfectionmagnetic nanoparticleshuman mesenchymal stem cellspolyethylenimineThe development of safe and efficient gene delivery systems is the key to the overall success of gene therapy. This work introduced a MNP-based nonviral gene delivery approach for transfection in clinically relevant cells. Moreover, the in vitro mechanism studies could explained the beneficial properties of the proposed carrier design. This work demonstrated, that proposed system could efficiently and safely transfect human bone marrow derived mesenchymal stem cells. Additionaly, the clinical perspectives of MNP-based gene therapy were discussed and evaluated in this dissertation.20172017[xslt]Saxonrosdok/id00001874https://purl.uni-rostock.de/rosdok/id0000187488299422010.18453/rosdok_id00001874urn:nbn:de:gbv:28-diss2017-0038-4Evgenya DelyaginaDelyagina EvgenyaMagnetic nanoparticle-based gene delivery : mechanisms and clinical perspectivesDissertationdoctoral thesisUniversität Rostock, 2017The development of safe and efficient gene delivery systems is the key to the overall success of gene therapy. This work introduced a MNP-based nonviral gene delivery approach for transfection in clinically relevant cells. Moreover, the in vitro mechanism studies could explained the beneficial…Evgenya DelyaginaMagnetic nanoparticle-based gene deliverymechanisms and clinical perspectivesMagnetische Nanopartikel-basierter GentransferMechanismen und Klinische PerspektivenUniversitätsbibliothek Rostockhttp://purl.uni-rostock.de/rosdok/id000018741128603144EvgenyaDelyagina1986-VerfasserInaut136129560Prof. Dr. med.GustavSteinhoffUniversitätsmedizin Rostock, Klinik und Poliklinik für HerzchirurgieAkademischeR BetreuerIndgs122722388Prof. Dr. med. Dr. rer. nat.Brigitte M.PützerUniversitätsmedizin Rostock, Institut für Experimentelle Gentherapie und TumorforschungAkademischeR BetreuerIndgs1082299979Prof. Dr. rer. nat.MartinZenkeRWTH Aachen, Universitätsklinikum der RWTH, Helmholtz-Institut für Biomedizinische TechnikAkademischeR BetreuerIndgs1029510660Universität RostockUniversitätsmedizinGrad-verleihende Institutiondgg[doi]10.18453/rosdok_id00001874[purl]http://purl.uni-rostock.de/rosdok/id00001874[urn]urn:nbn:de:gbv:28-diss2017-0038-4open_access20172017epoch:21th_centuryrfc5646:endoctype:epub.dissertationaccesscondition:openaccessSDNB:610institution:unirostock.umrstate:published