Wnt3a induces neuronal differentiation and crosstalk between Wnt/Ca2+-pathway and Wnt/β-catenin pathway

10.18453/rosdok_id00001884 Talabattula, Venkata Ajay Narendra Venkata Ajay Narendra Talabattula http://d-nb.info/gnd/1129883426 Wnt3a induces neuronal differentiation and crosstalk between Wnt/Ca2+-pathway and Wnt/β-catenin pathway Universität Rostock 2017 610 Medical sciences Medicine 2017 en http://purl.uni-rostock.de/rosdok/id00001884 urn:nbn:de:gbv:28-diss2017-0048-9 HumanNPCs (hNPCs) are the suitable cell source in translation medicine for neurodegenerative disease. In the present study, effects of Wnt3a on the differentiation of hNPC were studied. The results showed that Wnt3a induces canonical and non canonical Wnt pathways. Furthermore, Wnt3a increases Ca2+ target genes CaMKII and Pyk2 in the differentiating VM cells. The results demonstrated that Pyk2 decreases GSK3β activity and stabilizes β-catenin, suggesting there is an intercross between Wnt/β-catenin and Wnt/Ca2+-pathway via Pyk2.