10.18453/rosdok_id00001923
Ponndorf, Daniel Helmut
Daniel Helmut
Ponndorf
http://d-nb.info/gnd/1136290540
Increasing the Arginine content in plants by the coexpression of cyanophycin and cyanophycinase
Erhöhung des Arginingehaltes in Pflanzen durch die Koexpression von Cyanophycin und Cyanophycinase
Universität Rostock
2017
330 Economics
630 Agriculture
570 Life science
2017
en
http://purl.uni-rostock.de/rosdok/id00001923
urn:nbn:de:gbv:28-diss2017-0087-6
A new strategy for the increase of Arginine in plants was investigated, the Coexpression of Arg rich Cyanophycin (CGP) and its degrading enzyme Cyanophycinase (CGPase). The CGP production in plants was optimized for a commercial application and CGPase expression in plants established by transient expression. Enzyme and substrate could be coexpressed in one plant and CGP degradation was shown in plant material. This degradation was also shown in the intestine of mice, which did not lead to an increase of Arg, but of β-Asp-Arg dipeptides in the blood.