mycoreobjectrosdok_disshab_0000001866rosdok_disshab_0000001866rosdokdisshabrosdok_derivate_00000385832023-08-08T10:08:43.199Z2018-03-23T09:03:21.368ZadministratoreditorDpublished1rosdok_derivate_0000038583truetruederivate_types:fulltextVolltextfulltextwf_edit_epub wf_register_epubstate:publishedstate:publishedveröffentlichtpublishedrosdok/id00002044101834277XMODS updated during RosDok migration in June 2021DissertationHochschulschrift1124704744XiaoqiNi1984-VerfasserInautElectrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxideen123274303Prof. Dr.Gerd-UweFlechsigUniversity at Albany, SUNY, Dept. of ChemistryAkademischeR BetreuerIndgs132314614Prof. Dr.PeterLangerUniversity of Rostock, Dept. of ChemistryAkademischeR BetreuerIndgs2147083-2Universität RostockMathematisch-Naturwissenschaftliche FakultätGrad-verleihende Institutiondgg10.18453/rosdok_id00002044http://purl.uni-rostock.de/rosdok/id00002044urn:nbn:de:gbv:28-diss2018-0038-9540 ChemieMathematisch-Naturwissenschaftliche Fakultätfrei zugänglich (Open Access)Lizenz Metadaten: CC0Nutzungsrechte erteiltalle Rechte vorbehaltenUniversität RostockRostock2018monographic20172018Universitätsbibliothek RostockRostock20182018This dissertation describes the hybridization assay on gold electrodes for electrochemical detection of DNA and polymerase chain reaction (PCR) products by means of the electrochemically active compound osmium tetroxide bipyridine. Single base mismatches can be discriminated for their different thermal behavior. We further optimized protective-strand design, surface-probe density, and hybridization temperature. Besides, we also investigated to obtain labeled single stranded DNA targets from asymmetric PCR after labeling forward primer with osmium tetroxide for electrochemical detection.sandwich hybridizationgold electrodesingle base mismatchUniversitätsbibliothek Rostockhttp://purl.uni-rostock.de/rosdok/id00002044doctype:epubdoctype:epubDokumenttypDocument typedoctype:epub.dissertationdoctype:epub.dissertationDissertationdoctoral thesisdiniPublType:doctoralThesis diniPublType2022:PhDThesis XMetaDissPlusThesisLevel:thesis.doctoralinfo:eu-repo/semantics/doctoralThesisdocumentnatureOfContent:ppn_105825778natureOfContent:ppn_105825778HochschulschriftdiniPublType2022:DoctoralThesisdiniPublType2022:DoctoralThesisDissertation oder HabilitationDoctoral thesisDRIVERdiniPublType2022:PhDThesisdiniPublType2022:PhDThesisDissertationPhD thesisKDSF (Pu34)XMetaDissPlusThesisLevel:thesis.doctoralXMetaDissPlusThesisLevel:thesis.doctoralDoktorarbeitdoctoral thesisrfc5646:enrfc5646:enEnglischEnglishengengSDNB:540SDNB:540540 Chemie540 Chemistry & allied sciencesinstitution:unirostockinstitution:unirostockUniversität RostockUniversity of RostockUniversität RostockUniversität RostockUni.Rostockhttp://d-nb.info/gnd/38329-6institution:unirostock.mnfinstitution:unirostock.mnfMathematisch-Naturwissenschaftliche FakultätFaculty of Mathematics and Natural SciencesUniversität Rostock. Mathematisch-Naturwissenschaftliche FakultätMathematisch-Natur-<br />wissenschaftliche FakultätUni.Rostock.Fakultaet.MNFhttp://d-nb.info/gnd/2147083-2accesscondition:openaccessaccesscondition:openaccessfrei zugänglich (Open Access)open accesshttp://purl.org/coar/access_right/c_abf2OAfreeinfo:eu-repo/semantics/openAccess[DE-28]Open Access$gControlled Vocabulary for Access Rights$uhttp://purl.org/coar/access_right/c_abf2licenseinfo:metadatalicenseinfo:metadataLizenzen für Metadatenlicenseinfo:metadata.cc0licenseinfo:metadata.cc0Lizenz Metadaten: CC0license metadata: CC0/creativecommons/p/zero/1.0/88x31.pnghttps://creativecommons.org/publicdomain/zero/1.0/licenseinfo:depositlicenseinfo:depositVeröffentlichungsgenehmigungpermission to storelicenseinfo:deposit.rightsgrantedlicenseinfo:deposit.rightsgrantedNutzungsrechte erteiltrights grantedlicenseinfo:worklicenseinfo:workWerkworklicenseinfo:work.rightsreservedlicenseinfo:work.rightsreservedalle Rechte vorbehaltenall rights reserved/creativecommons/r/reserved/0.9/88x31.png[DE-28]Urheberrechtsschutz 1.0$gRights Statements$uhttp://rightsstatements.org/vocab/InC/1.0/http://rightsstatements.org/vocab/InC/1.0/http://rightsstatements.org/vocab/InC/1.0/Electrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxideElectrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxidegnd:1124704744gnd:123274303gnd:132314614gnd:2147083-2gnd:1124704744gnd:123274303gnd:132314614gnd:2147083-2rosdok_disshab_0000001866-d767510e39gnd:1124704744Xiaoqi NiXiaoqi Nirosdok_disshab_0000001866-d767510e60gnd:123274303Prof. Dr. Gerd-Uwe FlechsigProf. Dr. Gerd-Uwe Flechsigrosdok_disshab_0000001866-d767510e75gnd:132314614Prof. Dr. Peter LangerProf. Dr. Peter Langerrosdok_disshab_0000001866-d767510e90gnd:2147083-2Universität Rostock Mathematisch-Naturwissenschaftliche FakultätUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätXiaoqi NiProf. Dr. Gerd-Uwe FlechsigProf. Dr. Peter LangerUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätXiaoqi NiProf. Dr. Gerd-Uwe FlechsigProf. Dr. Peter LangerUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätXiaoqi NiRostockUniversität Rostockepub.dissertation10.18453/rosdok_id00002044http://purl.uni-rostock.de/rosdok/id00002044urn:nbn:de:gbv:28-diss2018-0038-9sandwich hybridizationgold electrodesingle base mismatchThis dissertation describes the hybridization assay on gold electrodes for electrochemical detection of DNA and polymerase chain reaction (PCR) products by means of the electrochemically active compound osmium tetroxide bipyridine. Single base mismatches can be discriminated for their different thermal behavior. We further optimized protective-strand design, surface-probe density, and hybridization temperature. Besides, we also investigated to obtain labeled single stranded DNA targets from asymmetric PCR after labeling forward primer with osmium tetroxide for electrochemical detection.20182018epub.dissertation1 Dissertation_Ni_2018.pdf 6829681 c36eee6dade005409b88488e73f9190f rosdok/id00002044101834277XMODS updated during RosDok migration in June 2021DissertationHochschulschrift1124704744XiaoqiNi1984-VerfasserInautElectrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxideen123274303Prof. Dr.Gerd-UweFlechsigUniversity at Albany, SUNY, Dept. of ChemistryAkademischeR BetreuerIndgs132314614Prof. Dr.PeterLangerUniversity of Rostock, Dept. of ChemistryAkademischeR BetreuerIndgs2147083-2Universität RostockMathematisch-Naturwissenschaftliche FakultätGrad-verleihende Institutiondgg10.18453/rosdok_id00002044http://purl.uni-rostock.de/rosdok/id00002044urn:nbn:de:gbv:28-diss2018-0038-9540 ChemieMathematisch-Naturwissenschaftliche Fakultätfrei zugänglich (Open Access)Lizenz Metadaten: CC0Nutzungsrechte erteiltalle Rechte vorbehaltenUniversität RostockRostock2018monographic20172018Universitätsbibliothek RostockRostock20182018This dissertation describes the hybridization assay on gold electrodes for electrochemical detection of DNA and polymerase chain reaction (PCR) products by means of the electrochemically active compound osmium tetroxide bipyridine. Single base mismatches can be discriminated for their different thermal behavior. We further optimized protective-strand design, surface-probe density, and hybridization temperature. Besides, we also investigated to obtain labeled single stranded DNA targets from asymmetric PCR after labeling forward primer with osmium tetroxide for electrochemical detection.sandwich hybridizationgold electrodesingle base mismatchUniversitätsbibliothek Rostockhttp://purl.uni-rostock.de/rosdok/id00002044 2018-03-23T09:03:21.368Z 2023-08-08T10:08:43.199Z 2023-08-18T10:08:43.204Z editorD {"identifier":"rosdok/id00002044","type":"local_id","additional":"","service":"MCRLocalID","created":"2018-06-30T13:34:25.409Z"} {"identifier":"http://purl.uni-rostock.de/rosdok/id00002044","type":"purl","additional":"","service":"RosDokPURL","created":"2018-06-30T13:34:25.612Z","registered":"2018-06-30T13:34:25.612Z"} {"identifier":"10.18453/rosdok_id00002044","type":"doi","additional":"","service":"RosDokDOI","created":"2018-06-30T13:34:27.027Z","registered":"2018-06-30T13:34:27.027Z"} {"identifier":"urn:nbn:de:gbv:28-diss2018-0038-9","type":"dnbUrn","additional":"","service":"RosDokURN","created":"2018-06-30T13:34:25.418Z","registered":"2018-04-12T02:25:38.120Z"} administratorfulltextfile/rosdok_disshab_0000001866/rosdok_derivate_0000038583/Dissertation_Ni_2018.pdfElectrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxideElectrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxidegnd:1124704744gnd:123274303gnd:132314614gnd:2147083-2gnd:1124704744gnd:123274303gnd:132314614gnd:2147083-2rosdok_disshab_0000001866-d767510e39gnd:1124704744Xiaoqi NiXiaoqi Nirosdok_disshab_0000001866-d767510e60gnd:123274303Prof. Dr. Gerd-Uwe FlechsigProf. Dr. Gerd-Uwe Flechsigrosdok_disshab_0000001866-d767510e75gnd:132314614Prof. Dr. Peter LangerProf. Dr. Peter Langerrosdok_disshab_0000001866-d767510e90gnd:2147083-2Universität Rostock Mathematisch-Naturwissenschaftliche FakultätUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätXiaoqi NiProf. Dr. Gerd-Uwe FlechsigProf. Dr. Peter LangerUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätXiaoqi NiProf. Dr. Gerd-Uwe FlechsigProf. Dr. Peter LangerUniversität Rostock Mathematisch-Naturwissenschaftliche FakultätXiaoqi NiRostockUniversität Rostockepub.dissertation10.18453/rosdok_id00002044http://purl.uni-rostock.de/rosdok/id00002044urn:nbn:de:gbv:28-diss2018-0038-9sandwich hybridizationgold electrodesingle base mismatchThis dissertation describes the hybridization assay on gold electrodes for electrochemical detection of DNA and polymerase chain reaction (PCR) products by means of the electrochemically active compound osmium tetroxide bipyridine. Single base mismatches can be discriminated for their different thermal behavior. We further optimized protective-strand design, surface-probe density, and hybridization temperature. Besides, we also investigated to obtain labeled single stranded DNA targets from asymmetric PCR after labeling forward primer with osmium tetroxide for electrochemical detection.20182018[xslt]Saxonrosdok/id00002044https://purl.uni-rostock.de/rosdok/id00002044101834277X10.18453/rosdok_id00002044urn:nbn:de:gbv:28-diss2018-0038-9Xiaoqi NiNi XiaoqiElectrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxideDissertationdoctoral thesisUniversität Rostock, 2018This dissertation describes the hybridization assay on gold electrodes for electrochemical detection of DNA and polymerase chain reaction (PCR) products by means of the electrochemically active compound osmium tetroxide bipyridine. Single base mismatches can be discriminated for their different…Xiaoqi NiElectrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxideUniversitätsbibliothek Rostockhttp://purl.uni-rostock.de/rosdok/id000020441124704744XiaoqiNi1984-VerfasserInaut123274303Prof. Dr.Gerd-UweFlechsigUniversity at Albany, SUNY, Dept. of ChemistryAkademischeR BetreuerIndgs132314614Prof. Dr.PeterLangerUniversity of Rostock, Dept. of ChemistryAkademischeR BetreuerIndgs2147083-2Universität RostockMathematisch-Naturwissenschaftliche FakultätGrad-verleihende Institutiondgg[doi]10.18453/rosdok_id00002044[purl]http://purl.uni-rostock.de/rosdok/id00002044[urn]urn:nbn:de:gbv:28-diss2018-0038-9open_access20182018epoch:21th_centuryrfc5646:endoctype:epub.dissertationaccesscondition:openaccessSDNB:540institution:unirostock.mnfstate:published