mycoreobjectrosdok_disshab_0000001878rosdok_disshab_0000001878rosdokdisshabrosdok_derivate_00000386332023-08-08T10:08:47.923Z2018-04-11T11:54:50.169ZadministratoreditorDpublished1rosdok_derivate_0000038633truetruederivate_types:fulltextVolltextfulltextwf_edit_epub wf_register_epubstate:publishedstate:publishedveröffentlichtpublishedrosdok/id000020561018502297MODS updated during RosDok migration in June 2021DissertationHochschulschrift1155939417NataliaVoronina1989-VerfasserInautProduction and characterization of genetically modified magnetized cells for future targeted treatment of cardiovascular diseasesen1068472898Prof. Dr.AlexanderPfeiferUniversitätsklinikum Bonn, Institut für Pharmakologie und ToxikologieAkademischeR BetreuerIndgsProf. Dr.RobertDavidUnversitätsmedizin Rostock, Referenz-und Translationszentrum für Kardiale StammzelltherapieAkademischeR BetreuerIndgsProf. Dr.Michael O.GlockerUnversitätsmedizin Rostock, ProteomzentrumAkademischeR BetreuerIndgs1029510660Universität RostockUniversitätsmedizinGrad-verleihende Institutiondgg10.18453/rosdok_id00002056http://purl.uni-rostock.de/rosdok/id00002056urn:nbn:de:gbv:28-diss2018-0050-4610 Medizin, GesundheitUniversitätsmedizinfrei zugänglich (Open Access)Lizenz Metadaten: CC0Nutzungsrechte erteiltalle Rechte vorbehaltenUniversität RostockRostock2018monographic20172018Universitätsbibliothek RostockRostock20182018Gene therapy is a promising tool for cardiovascular disease treatment. Its wide clinical application is, however, limited by the lack of suitable targeted gene delivery systems. In this work, a non-viral vector was tested - composed by polyethyleneimine and magnetic nanoparticles (PEI/MNP) - with primary endothelial cells as in vitro model for angiogenesis and CD133+ hematopoietic stem cells. The obtained results indicate that transient genetic modification of cells with miRNA delivered by PEI/MNP is feasible and results in their magnetization sufficient for targeting and MRI detection.gene therapystem cell engineeringmagnetically guided therapyUniversitätsbibliothek Rostockhttp://purl.uni-rostock.de/rosdok/id00002056doctype:epubdoctype:epubDokumenttypDocument typedoctype:epub.dissertationdoctype:epub.dissertationDissertationdoctoral thesisdiniPublType:doctoralThesis diniPublType2022:PhDThesis XMetaDissPlusThesisLevel:thesis.doctoralinfo:eu-repo/semantics/doctoralThesisdocumentnatureOfContent:ppn_105825778natureOfContent:ppn_105825778HochschulschriftdiniPublType2022:DoctoralThesisdiniPublType2022:DoctoralThesisDissertation oder HabilitationDoctoral thesisDRIVERdiniPublType2022:PhDThesisdiniPublType2022:PhDThesisDissertationPhD thesisKDSF (Pu34)XMetaDissPlusThesisLevel:thesis.doctoralXMetaDissPlusThesisLevel:thesis.doctoralDoktorarbeitdoctoral thesisrfc5646:enrfc5646:enEnglischEnglishengengSDNB:610SDNB:610610 Medizin, Gesundheit610 Medical sciences Medicineinstitution:unirostockinstitution:unirostockUniversität RostockUniversity of RostockUniversität RostockUniversität RostockUni.Rostockhttp://d-nb.info/gnd/38329-6institution:unirostock.umrinstitution:unirostock.umrUniversitätsmedizinUniversity MedicineUniversität Rostock. UniversitätsmedizinUniversitätsmedizinUni.Rostock.Fakultaet.MEDhttp://d-nb.info/gnd/1029510660accesscondition:openaccessaccesscondition:openaccessfrei zugänglich (Open Access)open accesshttp://purl.org/coar/access_right/c_abf2OAfreeinfo:eu-repo/semantics/openAccess[DE-28]Open Access$gControlled Vocabulary for Access Rights$uhttp://purl.org/coar/access_right/c_abf2licenseinfo:metadatalicenseinfo:metadataLizenzen für Metadatenlicenseinfo:metadata.cc0licenseinfo:metadata.cc0Lizenz Metadaten: CC0license metadata: CC0/creativecommons/p/zero/1.0/88x31.pnghttps://creativecommons.org/publicdomain/zero/1.0/licenseinfo:depositlicenseinfo:depositVeröffentlichungsgenehmigungpermission to storelicenseinfo:deposit.rightsgrantedlicenseinfo:deposit.rightsgrantedNutzungsrechte erteiltrights grantedlicenseinfo:worklicenseinfo:workWerkworklicenseinfo:work.rightsreservedlicenseinfo:work.rightsreservedalle Rechte vorbehaltenall rights reserved/creativecommons/r/reserved/0.9/88x31.png[DE-28]Urheberrechtsschutz 1.0$gRights Statements$uhttp://rightsstatements.org/vocab/InC/1.0/http://rightsstatements.org/vocab/InC/1.0/http://rightsstatements.org/vocab/InC/1.0/Production and characterization of genetically modified magnetized cells for future targeted treatment of cardiovascular diseasesProduction and characterization of genetically modified magnetized cells for future targeted treatment of cardiovascular diseasesgnd:1155939417gnd:1068472898gnd:1029510660gnd:1155939417gnd:1068472898gnd:1029510660rosdok_disshab_0000001878-d800670e39gnd:1155939417Natalia VoroninaNatalia Voroninarosdok_disshab_0000001878-d800670e60gnd:1068472898Prof. Dr. Alexander PfeiferProf. Dr. Alexander Pfeiferrosdok_disshab_0000001878-d800670e75Prof. Dr. Robert DavidProf. Dr. Robert Davidrosdok_disshab_0000001878-d800670e88Prof. Dr. Michael O. GlockerProf. Dr. Michael O. Glockerrosdok_disshab_0000001878-d800670e102gnd:1029510660Universität Rostock UniversitätsmedizinUniversität Rostock UniversitätsmedizinNatalia VoroninaProf. Dr. Alexander PfeiferProf. Dr. Robert DavidProf. Dr. Michael O. GlockerUniversität Rostock UniversitätsmedizinNatalia VoroninaProf. Dr. Alexander PfeiferProf. Dr. Robert DavidProf. Dr. Michael O. GlockerUniversität Rostock UniversitätsmedizinNatalia VoroninaRostockUniversität Rostockepub.dissertation10.18453/rosdok_id00002056http://purl.uni-rostock.de/rosdok/id00002056urn:nbn:de:gbv:28-diss2018-0050-4gene therapystem cell engineeringmagnetically guided therapyGene therapy is a promising tool for cardiovascular disease treatment. Its wide clinical application is, however, limited by the lack of suitable targeted gene delivery systems. In this work, a non-viral vector was tested - composed by polyethyleneimine and magnetic nanoparticles (PEI/MNP) - with primary endothelial cells as in vitro model for angiogenesis and CD133+ hematopoietic stem cells. The obtained results indicate that transient genetic modification of cells with miRNA delivered by PEI/MNP is feasible and results in their magnetization sufficient for targeting and MRI detection.20182018epub.dissertation1 Dissertation_Voronina_2018.pdf 9945000 b31cde4168bb9d839431b0cca830c1cd rosdok/id000020561018502297MODS updated during RosDok migration in June 2021DissertationHochschulschrift1155939417NataliaVoronina1989-VerfasserInautProduction and characterization of genetically modified magnetized cells for future targeted treatment of cardiovascular diseasesen1068472898Prof. Dr.AlexanderPfeiferUniversitätsklinikum Bonn, Institut für Pharmakologie und ToxikologieAkademischeR BetreuerIndgsProf. Dr.RobertDavidUnversitätsmedizin Rostock, Referenz-und Translationszentrum für Kardiale StammzelltherapieAkademischeR BetreuerIndgsProf. Dr.Michael O.GlockerUnversitätsmedizin Rostock, ProteomzentrumAkademischeR BetreuerIndgs1029510660Universität RostockUniversitätsmedizinGrad-verleihende Institutiondgg10.18453/rosdok_id00002056http://purl.uni-rostock.de/rosdok/id00002056urn:nbn:de:gbv:28-diss2018-0050-4610 Medizin, GesundheitUniversitätsmedizinfrei zugänglich (Open Access)Lizenz Metadaten: CC0Nutzungsrechte erteiltalle Rechte vorbehaltenUniversität RostockRostock2018monographic20172018Universitätsbibliothek RostockRostock20182018Gene therapy is a promising tool for cardiovascular disease treatment. Its wide clinical application is, however, limited by the lack of suitable targeted gene delivery systems. In this work, a non-viral vector was tested - composed by polyethyleneimine and magnetic nanoparticles (PEI/MNP) - with primary endothelial cells as in vitro model for angiogenesis and CD133+ hematopoietic stem cells. 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GlockerProf. Dr. Michael O. Glockerrosdok_disshab_0000001878-d800670e102gnd:1029510660Universität Rostock UniversitätsmedizinUniversität Rostock UniversitätsmedizinNatalia VoroninaProf. Dr. Alexander PfeiferProf. Dr. Robert DavidProf. Dr. Michael O. GlockerUniversität Rostock UniversitätsmedizinNatalia VoroninaProf. Dr. Alexander PfeiferProf. Dr. Robert DavidProf. Dr. Michael O. GlockerUniversität Rostock UniversitätsmedizinNatalia VoroninaRostockUniversität Rostockepub.dissertation10.18453/rosdok_id00002056http://purl.uni-rostock.de/rosdok/id00002056urn:nbn:de:gbv:28-diss2018-0050-4gene therapystem cell engineeringmagnetically guided therapyGene therapy is a promising tool for cardiovascular disease treatment. Its wide clinical application is, however, limited by the lack of suitable targeted gene delivery systems. In this work, a non-viral vector was tested - composed by polyethyleneimine and magnetic nanoparticles (PEI/MNP) - with primary endothelial cells as in vitro model for angiogenesis and CD133+ hematopoietic stem cells. The obtained results indicate that transient genetic modification of cells with miRNA delivered by PEI/MNP is feasible and results in their magnetization sufficient for targeting and MRI detection.20182018[xslt]Saxonrosdok/id00002056https://purl.uni-rostock.de/rosdok/id00002056101850229710.18453/rosdok_id00002056urn:nbn:de:gbv:28-diss2018-0050-4Natalia VoroninaVoronina NataliaProduction and characterization of genetically modified magnetized cells for future targeted treatment of cardiovascular diseasesDissertationdoctoral thesisUniversität Rostock, 2018Gene therapy is a promising tool for cardiovascular disease treatment. Its wide clinical application is, however, limited by the lack of suitable targeted gene delivery systems. In this work, a non-viral vector was tested - composed by polyethyleneimine and magnetic nanoparticles (PEI/MNP) - with…Natalia VoroninaProduction and characterization of genetically modified magnetized cells for future targeted treatment of cardiovascular diseasesUniversitätsbibliothek Rostockhttp://purl.uni-rostock.de/rosdok/id000020561155939417NataliaVoronina1989-VerfasserInaut1068472898Prof. Dr.AlexanderPfeiferUniversitätsklinikum Bonn, Institut für Pharmakologie und ToxikologieAkademischeR BetreuerIndgsProf. Dr.RobertDavidUnversitätsmedizin Rostock, Referenz-und Translationszentrum für Kardiale StammzelltherapieAkademischeR BetreuerIndgsProf. Dr.Michael O.GlockerUnversitätsmedizin Rostock, ProteomzentrumAkademischeR BetreuerIndgs1029510660Universität RostockUniversitätsmedizinGrad-verleihende Institutiondgg[doi]10.18453/rosdok_id00002056[purl]http://purl.uni-rostock.de/rosdok/id00002056[urn]urn:nbn:de:gbv:28-diss2018-0050-4open_access20182018epoch:21th_centuryrfc5646:endoctype:epub.dissertationaccesscondition:openaccessSDNB:610institution:unirostock.umrstate:published