10.18453/rosdok_id00004147 Baschung, Yannick Yannick Baschung http://d-nb.info/gnd/1280533463 https://orcid.org/0000-0003-2717-7706 Universität Rostock 2022 540 Chemistry & allied sciences 610 Medical sciences Medicine 2022 en http://purl.uni-rostock.de/rosdok/id00004147 urn:nbn:de:gbv:28-rosdok_id00004147-3 Affinity mass spectrometry using selective and pressure-enhanced proteolytic excision and extraction combined with MALDI and ESI-MS has been applied to the identification of epitope binding sites of GalNac and blood group oligosaccharides in lectins. The epitope peptides identified comprise all essential amino acids involved in carbohydrate recognition, in complete agreement with available X-ray structures. Coupling of a specific peptide glycoprobe to the gold surface of a biosensor chip was successfully employed for identification of carbohydrate epitopes and affinity measurements.