10.18453/rosdok_id00004885 Nissen, Laura Sofie Laura Sofie Nissen http://d-nb.info/gnd/1372417885 Universität Rostock 2023 500 Natural sciences 570 Life science 2023 en https://purl.uni-rostock.de/rosdok/id00004885 urn:nbn:de:gbv:28-rosdok_id00004885-1 This study focussed on the versatile AOR from Thermoanaerobacter sp. strain X514 (AORX514), which was biochemically characterized by producing a His-tagged version in Thermoanaerobacter kivui. The function of the enzyme was confirmed, as aldehyde-oxidation activity with the native redox partner ferredoxin, as well as with artificial redox partners. Optimization of the medium did increase specific activity of the heterologous protein, which is active over a wide temperature and pH range, and is able to oxidize a wide variety of aldehydes to their corresponding acids.