10.18453/rosdok_id00005005 Salti, Haitham Haitham Salti http://d-nb.info/gnd/1381808670 Universität Rostock 2024 570 Life science 2024 en https://purl.uni-rostock.de/rosdok/id00005005 urn:nbn:de:gbv:28-rosdok_id00005005-3 This study explores physical pretreatments to improve kidney decellularization, addressing the need for a bioartificial kidney as an alternative treatment for end-stage renal disease. Freezing-thawing cycles (FTC) and high hydrostatic pressure (HHP) were compared to chemical decellularization. FTC was the most effective in removing DNA and preserving the extracellular matrix (ECM), and showed better scaffold recellularization with kidney cells. Analysis with artificial intelligence revealed insights into the instructive memory of the ECM, guiding cells to avoid attachment to nonspecific sites.