| title: |
| Aqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based
ionic liquids |
|
| contributing persons: |
| Susanne Elisabeth Dreyer[VerfasserIn] |
 |
137450710 |
| Udo Kragl
, Prof. Dr.[AkademischeR BetreuerIn] |
| Uwe Bornscheuer
, Prof. Dr.[AkademischeR BetreuerIn] |
| Jürgen Hubbuch
, Prof. Dr.[AkademischeR BetreuerIn] |
|
| contributing corporate bodies: |
| Universität Rostock, Mathematisch-Naturwissenschaftliche Fakultät[Grad-verleihende Institution] |
|
2147083-2 |
|
| |
| abstract: |
|
Within the scope of this thesis, the application of ionic liquids (IL) for the generation
of aqueous two-phase systems (IL-based ATPS) and their use for the purification of
catalytically active enzymes was evaluated. By studying the partitioning of different
model proteins, a deeper understanding of the mechanisms influencing the partition
process is provided. The IL AmmoengTM 110 in combination with an inorganic salt (K2HPO4/KH2PO4)
can be used to establish an IL-based ATPS for the selective purification of two alcohol
dehydrogenases. The presence of IL within the system was found to be advantageous
due to its stability enhancing effect on both enzymes and its solubility enhancing
effect on hydrophobic substrates.
[English] |
|
| document type: |
|
| institution: |
| Faculty of Mathematics and Natural Sciences |
|
| language: |
|
| subject class (DDC): |
| 540 Chemistry & allied sciences |
|
| |
publication /
production: |
Rostock
|
Rostock: Universität Rostock
|
|
2008
|
|
| |
| identifiers: |
|
| |
| access condition: |
|
| license/rights statement: |
all rights reserved
This work may only be used under the terms of the German Copyright Law (Urheberrechtsgesetz). |
|
|
| RosDok id: |
rosdok_disshab_0000000182 |
| created / modified: |
27.02.2009 / 08.08.2023
|
| metadata license: |
The metadata of this document was dedicated to the public domain
(CC0 1.0 Universal Public Domain Dedication). |
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