Aqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquids

Susanne Elisabeth Dreyer

doi:10.18453/rosdok_id00000430

Susanne Elisabeth Dreyer

Aqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquids

Universität Rostock, 2008

https://doi.org/10.18453/rosdok_id00000430

Abstract: Within the scope of this thesis, the application of ionic liquids (IL) for the generation of aqueous two-phase systems (IL-based ATPS) and their use for the purification of catalytically active enzymes was evaluated. By studying the partitioning of different model proteins, a deeper understanding of the mechanisms influencing the partition process is provided. The IL AmmoengTM 110 in combination with an inorganic salt (K2HPO4/KH2PO4) can be used to establish an IL-based ATPS for the selective purification of two alcohol dehydrogenases. The presence of IL within the system was found to be advantageous due to its stability enhancing effect on both enzymes and its solubility enhancing effect on hydrophobic substrates.

Dissertation Freier Zugang

Titel:

Aqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquids

Beteiligte Personen:

Susanne Elisabeth Dreyer[VerfasserIn]
	137450710
Udo Kragl , Prof. Dr.[AkademischeR BetreuerIn]
Uwe Bornscheuer , Prof. Dr.[AkademischeR BetreuerIn]
Jürgen Hubbuch , Prof. Dr.[AkademischeR BetreuerIn]

Beteiligte Körperschaften:

Universität Rostock, Mathematisch-Naturwissenschaftliche Fakultät[Grad-verleihende Institution]
	2147083-2

Zusammenfassung:

Within the scope of this thesis, the application of ionic liquids (IL) for the generation of aqueous two-phase systems (IL-based ATPS) and their use for the purification of catalytically active enzymes was evaluated. By studying the partitioning of different model proteins, a deeper understanding of the mechanisms influencing the partition process is provided. The IL AmmoengTM 110 in combination with an inorganic salt (K2HPO4/KH2PO4) can be used to establish an IL-based ATPS for the selective purification of two alcohol dehydrogenases. The presence of IL within the system was found to be advantageous due to its stability enhancing effect on both enzymes and its solubility enhancing effect on hydrophobic substrates. [Englisch]

Dokumenttyp:

Dissertation

Einrichtung:

Mathematisch-Naturwissenschaftliche Fakultät

Sprache:

Englisch

Sachgruppe der DNB:

540 Chemie

Veröffentlichung /
Entstehung:

Rostock

Rostock: Universität Rostock

2008