Towards Electrochemical PCR-Chips : Contributions to Couple Enzymatic Amplification and Electrochemical Detection of DNA

Heiko Duwensee

doi:10.18453/rosdok_id00000504

Heiko Duwensee

Towards Electrochemical PCR-Chips : Contributions to Couple Enzymatic Amplification and Electrochemical Detection of DNA

Universität Rostock, 2009

https://doi.org/10.18453/rosdok_id00000504

Abstract: In this work the electrochemical DNA detection at heated electrodes as one alternative to the classical methods for the detection of DNA is presented. At elevated temperature an increase in sensitivity and selectivity as well as the minimization of electrode fouling could be observed. Surface modifications in the nano scale could further more improve the sensitivity. Procedures to detect genetically modified maize (MON 810) and PCR products were developed. The combination of DNA amplification and electrochemical detection was demonstrated.

doctoral thesis free access

title:

Towards Electrochemical PCR-Chips: Contributions to Couple Enzymatic Amplification and Electrochemical Detection of DNA

contributing persons:

Heiko Duwensee[VerfasserIn]
	138646740
Gerd-Uwe Flechsig , PD Dr.[AkademischeR BetreuerIn]
Joseph Wang , Prof. Dr.[AkademischeR BetreuerIn]
Frank-Michael Matysik , Prof. Dr.[AkademischeR BetreuerIn]

contributing corporate bodies:

Universität Rostock, Mathematisch-Naturwissenschaftliche Fakultät[Grad-verleihende Institution]
	2147083-2

abstract:

In this work the electrochemical DNA detection at heated electrodes as one alternative to the classical methods for the detection of DNA is presented. At elevated temperature an increase in sensitivity and selectivity as well as the minimization of electrode fouling could be observed. Surface modifications in the nano scale could further more improve the sensitivity. Procedures to detect genetically modified maize (MON 810) and PCR products were developed. The combination of DNA amplification and electrochemical detection was demonstrated. [English]

In dieser Arbeit wird die elektrochemische DNA-Detektion an geheizten Elektroden als Alternative zu den klassischen Detektionsmethoden vorgestellt. Messungen bei erhöhten Temperaturen zeigten eine Verbesserung der Empfindlichkeit und der Spezifität sowie eine Minimierung des Elektrodenfoulings. Oberflächenmodifikationen im Nanomaßstab konnten die Empfindlichkeit weiter verbessern. Methoden, um gentechnisch veränderten Mais (MON 810) und PCR Produkte nachzuweisen, wurden vorgestellt. Die Kombination aus DNA-Vervielfältigung und elektrochemischer Detektion wurde erfolgreich angewendet. [German]

document type:

doctoral thesis

institution:

Faculty of Mathematics and Natural Sciences

language:

English

subject class (DDC):

540 Chemistry & allied sciences

publication /
production:

Rostock: Universität Rostock

2009

identifiers: