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Heiko Duwensee

Towards Electrochemical PCR-Chips : Contributions to Couple Enzymatic Amplification and Electrochemical Detection of DNA

Universität Rostock, 2009

Abstract: In this work the electrochemical DNA detection at heated electrodes as one alternative to the classical methods for the detection of DNA is presented. At elevated temperature an increase in sensitivity and selectivity as well as the minimization of electrode fouling could be observed. Surface modifications in the nano scale could further more improve the sensitivity. Procedures to detect genetically modified maize (MON 810) and PCR products were developed. The combination of DNA amplification and electrochemical detection was demonstrated.

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