Universität Rostock, 2010
Abstract: Mirabilis jalapa displays a strongly regulated flower opening process and emission of the floral volatile (E)-β-ocimene, which is produced by the (E)-β-ocimene synthase ((E)-β-OS). This enzyme could be characterized in flowers of Mirabilis jalapa. It is a single-product monoterpene synthase monomer of approximately 80 kDa with a high temperature tolerance. The in vivo activity of the (E)-β-OS correlates with the emission of (E)-β-ocimene and the content of the volatile in the emitting tissue. Histological analyses showed no developmentally regulated accumulation of monoterpene in the emitting tissue. This indicates a process of simultaneous synthesis and emission of (E)-β-ocimene.
doctoral thesis free access