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Tobias Latzkow

Einfluss von untranslatierten Regionen und Terminatoren auf die Expression von Transgenen unter subletalen Temperaturstress

Universität Rostock, 2011

Abstract: Under moderate heat stress conditions the combination of the 5' untranslated region of Phosphinothricin-N-acetyltransferase gene from Streptomyces viridochromogenes with the 3' termination signal of nopalin synthase gene of Agrobacterium tumefaciens mediate a inactivation of the transgene in herbicide resistant Nicotiana tabacum and Arabidopsis thaliana (Col-0) plants. A fusion of the luciferase coding region (luc) from Photinus pyralis with these specific elements is similarly inactivated in transgenic tobacco plants.

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