Genetic characterization, heterologous expression and application of S-layer proteins from the bacterial isolates Lysinibacillus sphaericus JG-B53 and Lysinibacillus sphaericus JG-A12

Franziska Lederer

doi:10.18453/rosdok_id00001119

Franziska Lederer

Genetic characterization, heterologous expression and application of S-layer proteins from the bacterial isolates Lysinibacillus sphaericus JG-B53 and Lysinibacillus sphaericus JG-A12

Universität Rostock, 2012

https://doi.org/10.18453/rosdok_id00001119

Abstract: The bacterial isolates Lysinibacillus sphaericus JG-A12 and JG-B53 were isolated from a uranium mining waste pile that is contaminated with multiple heavy metal ions. Genome analyses of Bacillus sp. JG-B53 were used to identify bacterial strategies to handle heavy metal polluted environments. The heterologous expression of the silent S-layer like protein gene sllB of L. sphaericus JG-A12 in E. coli induced distinct morphological modifications. Investigations identified SllB, to inhibit bacterial cell division. The filamentous structures were used for the design of polyelectrolyte capsules.

doctoral thesis free access

title:

Genetic characterization, heterologous expression and application of S-layer proteins from the bacterial isolates Lysinibacillus sphaericus JG-B53 and Lysinibacillus sphaericus JG-A12

other title:

Genetische Charakterisierung, heterologe Expression und die Verwendung von S-Layer Proteinen der bakteriellen Isolate Lysinibacillus sphaericus JG-B53 und Lysinibacillus sphaericus JG-A12 [translated]

contributing persons:

Franziska Lederer[VerfasserIn]
	1032376813
Hubert Bahl , Prof. Dr. rer. nat.[AkademischeR BetreuerIn]
Erika Kothe , Prof. Dr. rer. nat.[AkademischeR BetreuerIn]

contributing corporate bodies:

Universität Rostock, Mathematisch-Naturwissenschaftliche Fakultät[Grad-verleihende Institution]
	2147083-2

abstract:

The bacterial isolates Lysinibacillus sphaericus JG-A12 and JG-B53 were isolated from a uranium mining waste pile that is contaminated with multiple heavy metal ions. Genome analyses of Bacillus sp. JG-B53 were used to identify bacterial strategies to handle heavy metal polluted environments. The heterologous expression of the silent S-layer like protein gene sllB of L. sphaericus JG-A12 in E. coli induced distinct morphological modifications. Investigations identified SllB, to inhibit bacterial cell division. The filamentous structures were used for the design of polyelectrolyte capsules. [English]

Die bakteriellen Isolate Lysinibacillus sphaericus JG-A12 und JG-B53 wurden aus einer mit Schwermetallen kontaminierten Uranabfallhalde isoliert. Genomanalysen von Bacillus sp. JG-B53 wurden genutzt, um bakterielle Strategien zum Umgang mit schwermetallkontaminierten Lebensräumen zu identifizieren. Die heterologe Expression des S-Layer ähnlichen Proteins sllB von L. sphaericus JG-A12 in E. coli induzierte deutliche morphologische Veränderung. Untersuchungen identifizierten SllB als Ursache für eine gehemmte Zellteilung. Die Zellen wurden zur Herstellung von Polyelektrolytkapseln verwendet. [German]

document type:

doctoral thesis

institution:

Faculty of Mathematics and Natural Sciences

language:

English

subject class (DDC):

570 Life science

publication /
production:

Rostock: Universität Rostock

2012

identifiers: