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Diana Pohle

Steigerung der Matrixproduktion von humanen artikulären Chondrozyten durch eine Kombination aus adenoviraler Transduktion, Expansion im Monolayer und 3D-Kultur in Alginat

Universität Rostock, 2013

Abstract: Treatment of human articular chondrocytes (healthy or with low osteoarthritis score) with a combination of adenoviral transduction, monolayer expansion and cultivation in alginate beads, as during autologous chondrocyte implantation, led to an increased expression of anabolic genes and an augmented matrix production. The success of adenoviral gene therapy is dependent on the patient's immune response to the virus - no or only slight reaction is required. Infection and neutralisation test were carried out with various types of adenvirus, the most effective to be shown was adenovirus type 34.

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