title: |
Biochemical analysis of the alpha-terpineol/1,8-cineole cyclization reaction of the
cineole synthase of Nicotiana suaveolens |
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other title: |
Biochemische Analyse der alpha-Terpinol/1,8-Cineol Zyklisierungsreaktion der Cineolsynthase
aus Nicotiana suaveolens
[translated]
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contributing persons: |
Anne Brosemann[VerfasserIn] |
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1080058109 |
Birgit Piechulla
, Porf. Dr.[AkademischeR BetreuerIn] |
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131807838 |
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Universität Rostock, Institut für Biowissenschaften, Abteilung Biochemie |
Jörg Degenhardt
, Prof. Dr.[AkademischeR BetreuerIn] |
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Martin-Luther-Universität Halle-Wittenberg, Arbeitsgruppe Pharmazeutische Biotechnologie |
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contributing corporate bodies: |
Universität Rostock, Mathematisch-Naturwissenschaftliche Fakultät[Grad-verleihende Institution] |
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2147083-2 |
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abstract: |
19 mutants of the cineole synthase of N. suaveolens were generated by site-directed
mutagenesis in order to substitute the amino acids of the cineole synthase with amino
acids of the equivalent position of the terpineol synthase of N. langsdorfii. The
mutant enzyme F266S carrying a phenylalanine replacement by serine showed an inverted
product spectrum compared to the wild type enzyme. Terpineol was the major compound
in the product profile of the mutant F266S. It is hypothesized that the synthesis
of cineole proceeds independent of terpineol and via α-terpinyl hydronium ion (Wise
et al. 2002).
[English] |
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document type: |
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institution: |
Faculty of Mathematics and Natural Sciences |
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language: |
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subject class (DDC): |
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publication / production: |
Rostock
Rostock: Universität Rostock
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2015
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identifiers: |
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access condition: |
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license/rights statement: |
all rights reserved This work may only be used under the terms of the German Copyright Law (Urheberrechtsgesetz). |
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RosDok id: |
rosdok_disshab_0000001479 |
created / modified: |
11.12.2015 / 08.08.2023
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metadata license: |
The metadata of this document was dedicated to the public domain (CC0 1.0 Universal Public Domain Dedication). |