title: |
MicroRNA-based stem cell modifications using magnetic transfection complexes: A non-viral
approach towards cardiac regeneration |
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other title: |
MikroRNA-basierte Stammzellmodifikationen mittels magnetischer Transfektionskomplexe:
Ein nicht-viraler Ansatz für die kardiale Regeneration
[translated]
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contributing persons: |
Anna Schade[VerfasserIn] |
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108177682X |
Gustav Steinhoff
, Prof. Dr. med.[AkademischeR BetreuerIn] |
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136129560 |
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Universitätsmedizin Rostock, Klinik und Poliklinik für Herzchirurgie |
Brigitte M. Pützer
, Prof. Dr. med. Dr. rer. nat.[AkademischeR BetreuerIn] |
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122722388 |
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Universitätsmedizin Rostock, Institut für Experimentelle Gentherapie und Tumorforschung |
Stefan Brunner
, PD Dr. med.[AkademischeR BetreuerIn] |
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Universität München, Medizinische Klinik und Poliklinik I |
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contributing corporate bodies: |
Universität Rostock, Universitätsmedizin[Grad-verleihende Institution] |
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1029510660 |
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abstract: |
Regenerative properties of human mesenchymal stem cells (hMSC) can be enhanced by
genetic modifications. Thus we aimed to develop a magnetic non-viral vector for microRNA
(miR) delivery in freshly isolated hMSCs. miR/PEI/MNP transfection showed a better
long term performance compared to miR/PEI transfection. Moreover magnetically modified
hMSCs could be guided by an external magnet contributing to enhanced cell engraftment.
Conclusively a magnetic non-viral carrier for efficient miR delivery in freshly isolated
hMSCs was developed allowing specific control of stem cells and magnetic targeting.
[English] |
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document type: |
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institution: |
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language: |
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subject class (DDC): |
610 Medical sciences Medicine |
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publication / production: |
Rostock
Rostock: Universität Rostock
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2015
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identifiers: |
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access condition: |
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license/rights statement: |
all rights reserved This work may only be used under the terms of the German Copyright Law (Urheberrechtsgesetz). |
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RosDok id: |
rosdok_disshab_0000001505 |
created / modified: |
22.12.2015 / 08.08.2023
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metadata license: |
The metadata of this document was dedicated to the public domain (CC0 1.0 Universal Public Domain Dedication). |