Influence of polymer surface modification and specific growth factor stimulation on cellular adhesion at the implant/endothelium interface

Thilo Storm

doi:10.18453/rosdok_id00001772

Thilo Storm

Influence of polymer surface modification and specific growth factor stimulation on cellular adhesion at the implant/endothelium interface

Universität Rostock, 2016

https://doi.org/10.18453/rosdok_id00001772

Abstract: The response of human endothelial cells (ECs) to poly(ε-caprolactone) (PCL) and eight different PCL modifications was examined in vitro. In CLSM, cell proliferation and viability testing, ECs responded best to plasma chemical activation of PCL. When ECs were additionally stimulated by VEGF, their maximum response was again seen on plasma activated PCL. Polymer precoating with matrix proteins was found to be beneficial for EC adhesion. A qPCR study of eNOS, IL-8, ICAM-1, PECAM-1 and VCAM-1 transcripts after VEGF stimulation of ECs on four modified and coated PCL surfaces was also performed.

Dissertation Freier Zugang

Titel:

Influence of polymer surface modification and specific growth factor stimulation on cellular adhesion at the implant/endothelium interface

Beteiligte Personen:

Thilo Storm[VerfasserIn]
	1074969685
Birgit Piechulla , Prof. Dr.[AkademischeR BetreuerIn]
	131807838
	Universität Rostock, Institut für Biowissenschaften
Marina Hovakimyan , PD Dr.[AkademischeR BetreuerIn]
	138627258
	Universität Rostock, Institut für Biomedizinische Technik
Mathias Wilhelmi , Prof. Dr.[AkademischeR BetreuerIn]
	120982145
	Medizinische Hochschule Hannover, Klinik für Herz-, Thorax-, Transplantations- und Gefäßchirurgie

Beteiligte Körperschaften:

Universität Rostock, Mathematisch-Naturwissenschaftliche Fakultät[Grad-verleihende Institution]
	2147083-2

Zusammenfassung:

The response of human endothelial cells (ECs) to poly(ε-caprolactone) (PCL) and eight different PCL modifications was examined in vitro. In CLSM, cell proliferation and viability testing, ECs responded best to plasma chemical activation of PCL. When ECs were additionally stimulated by VEGF, their maximum response was again seen on plasma activated PCL. Polymer precoating with matrix proteins was found to be beneficial for EC adhesion. A qPCR study of eNOS, IL-8, ICAM-1, PECAM-1 and VCAM-1 transcripts after VEGF stimulation of ECs on four modified and coated PCL surfaces was also performed. [Englisch]

Dokumenttyp:

Dissertation

Einrichtung:

Mathematisch-Naturwissenschaftliche Fakultät

Sprache:

Englisch

Sachgruppe der DNB:

570 Biowissenschaften, Biologie

Veröffentlichung /
Entstehung:

Rostock

Rostock: Universität Rostock

2016