Universität Rostock, 2016
Abstract: 1,4-Butanediol (1,4-BDO) is an important commodity chemical with various technical applications, within the production of plastics, polyesters and spandex fibers. 1,4-BDO is not a natural product and is exclusively produced by chemical petroleum-based synthesis. The objective of this work was the development of a strategy for the biological 1,4-BDO production from renewable resources using the host organism Clostridium acetobutylicum. For this, a new branch in the metabolism of C. acetobutylicum was generated by introducing a heterologously expressed 4-Hydroxybutyryl-CoA dehydratase (4HBD).
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