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Thomas Josef Dauben

Establishment of a novel in vitro implant infection model : effects of electrical stimulation on staphylococci using alternating current

Universität Rostock, 2018

https://doi.org/10.18453/rosdok_id00002037

Abstract: An in vitro system for electrical stimulation of cells and bacteria was developed. Stimulation of cells and bacteria can be conducted under similar conditions. Prior to experiments, numerical simulations were performed. Methods for evaluation of effects were established. Stimulation showed significant effects on bacterial growth and biofilm mass production. Following stimulation, changes in extracellular DNA amounts were detected. In co-culture, stimulation led to increased CFU/ml S. epidermidis in supernatants, on and into MG63 cells on electrode surfaces and chamber bottom.

doctoral thesis   free access