Establishment of a novel in vitro implant infection model : effects of electrical stimulation on staphylococci using alternating current

Thomas Josef Dauben

doi:10.18453/rosdok_id00002037

Thomas Josef Dauben

Establishment of a novel in vitro implant infection model : effects of electrical stimulation on staphylococci using alternating current

Universität Rostock, 2018

https://doi.org/10.18453/rosdok_id00002037

Abstract: An in vitro system for electrical stimulation of cells and bacteria was developed. Stimulation of cells and bacteria can be conducted under similar conditions. Prior to experiments, numerical simulations were performed. Methods for evaluation of effects were established. Stimulation showed significant effects on bacterial growth and biofilm mass production. Following stimulation, changes in extracellular DNA amounts were detected. In co-culture, stimulation led to increased CFU/ml S. epidermidis in supernatants, on and into MG63 cells on electrode surfaces and chamber bottom.

Dissertation Freier Zugang

Titel:

Establishment of a novel in vitro implant infection model: effects of electrical stimulation on staphylococci using alternating current

Beteiligte Personen:

Thomas Josef Dauben[VerfasserIn]
	1154729249
Bernd Kreikemeyer , Prof. Dr. rer. nat.[AkademischeR BetreuerIn]
	114684618
	Universitätsmedizin Rostock, Institut für Medizinische Mikrobiologie, Virologie und Hygiene
Hubert Bahl , Prof. Dr. rer. nat.[AkademischeR BetreuerIn]
	132475170
	Universität Rostock, Institut für Biowissenschaften
Rainer Bader , Prof. Dr. med.[AkademischeR BetreuerIn]
	133499847
	Universitätsmedizin Rostock, Orthopädische Klinik und Poliklinik, Forschungslabor für Biomechanik und Implantattechnologie

Beteiligte Körperschaften:

Universität Rostock, Mathematisch-Naturwissenschaftliche Fakultät[Grad-verleihende Institution]
	2147083-2

Zusammenfassung:

An in vitro system for electrical stimulation of cells and bacteria was developed. Stimulation of cells and bacteria can be conducted under similar conditions. Prior to experiments, numerical simulations were performed. Methods for evaluation of effects were established. Stimulation showed significant effects on bacterial growth and biofilm mass production. Following stimulation, changes in extracellular DNA amounts were detected. In co-culture, stimulation led to increased CFU/ml S. epidermidis in supernatants, on and into MG63 cells on electrode surfaces and chamber bottom. [Englisch]

Dokumenttyp:

Dissertation

Einrichtung:

Mathematisch-Naturwissenschaftliche Fakultät

Sprache:

Englisch

Sachgruppe der DNB:

570 Biowissenschaften, Biologie

Veröffentlichung /
Entstehung:

Rostock

Rostock: Universität Rostock

2018