Production and characterization of genetically modified magnetized cells for future targeted treatment of cardiovascular diseases

Natalia Voronina

doi:10.18453/rosdok_id00002056

Natalia Voronina

Production and characterization of genetically modified magnetized cells for future targeted treatment of cardiovascular diseases

Universität Rostock, 2018

https://doi.org/10.18453/rosdok_id00002056

Abstract: Gene therapy is a promising tool for cardiovascular disease treatment. Its wide clinical application is, however, limited by the lack of suitable targeted gene delivery systems. In this work, a non-viral vector was tested - composed by polyethyleneimine and magnetic nanoparticles (PEI/MNP) - with primary endothelial cells as in vitro model for angiogenesis and CD133+ hematopoietic stem cells. The obtained results indicate that transient genetic modification of cells with miRNA delivered by PEI/MNP is feasible and results in their magnetization sufficient for targeting and MRI detection.

Dissertation Freier Zugang

Titel:

Production and characterization of genetically modified magnetized cells for future targeted treatment of cardiovascular diseases

Beteiligte Personen:

Natalia Voronina[VerfasserIn]
	1155939417
Alexander Pfeifer , Prof. Dr.[AkademischeR BetreuerIn]
	1068472898
	Universitätsklinikum Bonn, Institut für Pharmakologie und Toxikologie
Robert David , Prof. Dr.[AkademischeR BetreuerIn]
	Unversitätsmedizin Rostock, Referenz-und Translationszentrum für Kardiale Stammzelltherapie
Michael O. Glocker , Prof. Dr.[AkademischeR BetreuerIn]
	Unversitätsmedizin Rostock, Proteomzentrum

Beteiligte Körperschaften:

Universität Rostock, Universitätsmedizin[Grad-verleihende Institution]
	1029510660

Zusammenfassung:

Gene therapy is a promising tool for cardiovascular disease treatment. Its wide clinical application is, however, limited by the lack of suitable targeted gene delivery systems. In this work, a non-viral vector was tested - composed by polyethyleneimine and magnetic nanoparticles (PEI/MNP) - with primary endothelial cells as in vitro model for angiogenesis and CD133+ hematopoietic stem cells. The obtained results indicate that transient genetic modification of cells with miRNA delivered by PEI/MNP is feasible and results in their magnetization sufficient for targeting and MRI detection. [Englisch]

Dokumenttyp:

Dissertation

Einrichtung:

Universitätsmedizin

Sprache:

Englisch

Sachgruppe der DNB:

610 Medizin, Gesundheit

Veröffentlichung /
Entstehung:

Rostock

Rostock: Universität Rostock

2018