title: |
Investigating the mechanism of a novel glycine-dependent riboswitch and a putative
non-coding regulatory RNA in Streptococcus pyogenes |
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contributing persons: |
Afsaneh Khani[VerfasserIn] |
|
1161756558 |
Bernd Kreikemeyer
, Prof. Dr.[AkademischeR BetreuerIn] |
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114684618 |
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Universitätsmedizin Rostock, Institut für Medizinische Mikrobiologie, Virologie und
Hygiene |
Hubert Bahl
, Prof. Dr.[AkademischeR BetreuerIn] |
|
132475170 |
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Universität Rostock, Institut für Biowissenschaften |
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contributing corporate bodies: |
Universität Rostock, Mathematisch-Naturwissenschaftliche Fakultät[Grad-verleihende Institution] |
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2147083-2 |
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abstract: |
We investigated gene expression regulation by a putative glycine(Gly) riboswitch located
in the 5′-UTR of a SAF protein gene in the S.pyogenes. Gly-dependency was studied
using a luciferase reporter gene system. Maximal reporter gene expression happened
in the presence of low Gly concentrations. RT-qPCR showed that in the presence of
Gly (≥1 mM), expression of the gene was downregulated. Growth in the presence of 0.1
mM Gly led to the production of a full-length transcript. We conclude that the Gly
riboswitch in S. pyogenes represses gene expression in the presence of high Gly concentrations.
[English] |
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document type: |
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institution: |
Faculty of Mathematics and Natural Sciences |
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language: |
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subject class (DDC): |
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publication / production: |
Rostock
Rostock: Universität Rostock
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2018
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identifiers: |
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access condition: |
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license/rights statement: |
all rights reserved This work may only be used under the terms of the German Copyright Law (Urheberrechtsgesetz). |
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RosDok id: |
rosdok_disshab_0000001915 |
created / modified: |
26.06.2018 / 08.08.2023
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metadata license: |
The metadata of this document was dedicated to the public domain (CC0 1.0 Universal Public Domain Dedication). |