Investigating the mechanism of a novel glycine-dependent riboswitch and a putative non-coding regulatory RNA in Streptococcus pyogenes

Afsaneh Khani

doi:10.18453/rosdok_id00002093

Afsaneh Khani

Investigating the mechanism of a novel glycine-dependent riboswitch and a putative non-coding regulatory RNA in Streptococcus pyogenes

Universität Rostock, 2018

https://doi.org/10.18453/rosdok_id00002093

Abstract: We investigated gene expression regulation by a putative glycine(Gly) riboswitch located in the 5′-UTR of a SAF protein gene in the S.pyogenes. Gly-dependency was studied using a luciferase reporter gene system. Maximal reporter gene expression happened in the presence of low Gly concentrations. RT-qPCR showed that in the presence of Gly (≥1 mM), expression of the gene was downregulated. Growth in the presence of 0.1 mM Gly led to the production of a full-length transcript. We conclude that the Gly riboswitch in S. pyogenes represses gene expression in the presence of high Gly concentrations.

Dissertation Freier Zugang

Titel:

Investigating the mechanism of a novel glycine-dependent riboswitch and a putative non-coding regulatory RNA in Streptococcus pyogenes

Beteiligte Personen:

Afsaneh Khani[VerfasserIn]
	1161756558
Bernd Kreikemeyer , Prof. Dr.[AkademischeR BetreuerIn]
	114684618
	Universitätsmedizin Rostock, Institut für Medizinische Mikrobiologie, Virologie und Hygiene
Hubert Bahl , Prof. Dr.[AkademischeR BetreuerIn]
	132475170
	Universität Rostock, Institut für Biowissenschaften

Beteiligte Körperschaften:

Universität Rostock, Mathematisch-Naturwissenschaftliche Fakultät[Grad-verleihende Institution]
	2147083-2

Zusammenfassung:

We investigated gene expression regulation by a putative glycine(Gly) riboswitch located in the 5′-UTR of a SAF protein gene in the S.pyogenes. Gly-dependency was studied using a luciferase reporter gene system. Maximal reporter gene expression happened in the presence of low Gly concentrations. RT-qPCR showed that in the presence of Gly (≥1 mM), expression of the gene was downregulated. Growth in the presence of 0.1 mM Gly led to the production of a full-length transcript. We conclude that the Gly riboswitch in S. pyogenes represses gene expression in the presence of high Gly concentrations. [Englisch]

Dokumenttyp:

Dissertation

Einrichtung:

Mathematisch-Naturwissenschaftliche Fakultät

Sprache:

Englisch

Sachgruppe der DNB:

570 Biowissenschaften, Biologie

Veröffentlichung /
Entstehung:

Rostock

Rostock: Universität Rostock

2018