| title: |
| Serodiagnosis of influenza virus and Newcastle disease virus: new molecular approaches |
|
| contributing persons: |
| Na Zhao[VerfasserIn] |
 |
1295109409 |
| Helen Louton[AkademischeR BetreuerIn] |
|
Universität Rostock, AUF, Tiergesundheit und Tierschutz |
| Timm C. Harder[AkademischeR BetreuerIn] |
 |
0000-0003-2387-378X |
|
1019544198 |
| Martin Pfeffer[AkademischeR BetreuerIn] |
|
1244188859 |
|
Universität Leipzig, FB Epidemiologie |
|
| contributing corporate bodies: |
| Universität Rostock[Grad-verleihende Institution] |
|
38329-6 |
| Universität Rostock, Agrar- und Umweltwissenschaftliche Fakultät[Grad-verleihende Institution] |
|
10025954-6 |
|
| |
| abstract: |
|
New serodiagnostic formats for the detection of influenza virus and NDV infections
were established and validated. Here, several recombinant proteins of influenza A
virus and ND virus were expressed via bacterial expression systems. Authentic folding
of these proteins was achieved in vitro. These recombinant proteins served as antigens
in indirect ELISA assays or in Luminex bead-based liquid arrays. It is demonstrated
that serological assays based on these recombinant proteins are potential alternative
methods to classical yet difficult to standardize hemagglutination inhibition assays.
[English] |
|
Es wurden mehrere rekombinante Proteine des Influenza-A-Virus und des ND-Virus in
einem bakteriellen Expressionssystem produziert und durch weitere In-vitro-Verfahren
authentisch gefaltet. Die rekombinanten Proteine wurden als Antigene in indirekten
ELISA-Assays oder in sogenannten Luminex-Bead “liquid arrays” evaluiert. Es konnte
gezeigt werden, dass serologische Assays auf Basis dieser rekombinanten Proteine eine
mögliche alternative Methode zu den klassischen, jedoch schwer standardisierbaren,
Hemagglutinationsinhibitionsverfahren sind.
[German] |
|
| document type: |
|
| institution: |
| Faculty of Agricultural and Environmental Sciences |
|
| language: |
|
| subject class (DDC): |
| 570 Life science |
| 630 Agriculture |
|
| |
publication /
production: |
Rostock
|
Rostock: Universität Rostock
|
|
2022
|
|
| statement of responsibility: |
|
| notes: |
| Enthält Zeitschriftenartikel |
|
| |
| identifiers: |
|
| |
| access condition: |
|
| license/rights statement: |
|
|
| RosDok id: |
rosdok_disshab_0000003000 |
| created / modified: |
07.07.2023 / 08.08.2023
|
| metadata license: |
The metadata of this document was dedicated to the public domain
(CC0 1.0 Universal Public Domain Dedication). |
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