Elucidation of molecular recognition structures of peptides and proteins to antibodies and DNA aptamers by biosensor- mass spectrometry combination

Loredana-Mirela Lupu

doi:10.18453/rosdok_id00004458

Loredana-Mirela Lupu

Elucidation of molecular recognition structures of peptides and proteins to antibodies and DNA aptamers by biosensor- mass spectrometry combination

Universität Rostock, 2022

https://doi.org/10.18453/rosdok_id00004458

Abstract: The PROTEX-SPR-MS method has unveiled several epitopes with promising medical applications, including an epitope peptide suitable for antibody removal in Fabry's disease. Additionally, an epitope conducive to early heart muscle injury diagnosis was identified and characterized.Furthermore, PROTEX-SPR-MS has been employed to study two distinct single-stranded DNA aptamers interacting with a tumor biomarker protein. This analysis has revealed that aptamers have remarkably similar affinity and epitope binding to antibodies, suggesting their potential utility in medical applications.

Dissertation Freier Zugang

Titel:

Elucidation of molecular recognition structures of peptides and proteins to antibodies and DNA aptamers by biosensor- mass spectrometry combination

Beteiligte Personen:

Loredana-Mirela Lupu[VerfasserIn]
	0009-0003-5299-0907
	1309108412
Markus Tiedge[AkademischeR BetreuerIn]
	13860231X
	Institut für Medizinische Biochemie und Molekularbiologie
Michael Glocker[AkademischeR BetreuerIn]
	0000-0001-9190-482X
	1014052165
Michael Przybylski[AkademischeR BetreuerIn]
	1130680797
	AffyMSLifeChem

Beteiligte Körperschaften:

Universität Rostock[Grad-verleihende Institution]
	38329-6
Universitätsmedizin Rostock[Grad-verleihende Institution]
	1029510660

Zusammenfassung:

The PROTEX-SPR-MS method has unveiled several epitopes with promising medical applications, including an epitope peptide suitable for antibody removal in Fabry's disease. Additionally, an epitope conducive to early heart muscle injury diagnosis was identified and characterized.Furthermore, PROTEX-SPR-MS has been employed to study two distinct single-stranded DNA aptamers interacting with a tumor biomarker protein. This analysis has revealed that aptamers have remarkably similar affinity and epitope binding to antibodies, suggesting their potential utility in medical applications. [Englisch]

Dokumenttyp:

Dissertation

Einrichtung:

Universitätsmedizin

Sprache:

Englisch

Sachgruppe der DNB:

500 Naturwissenschaften

Veröffentlichung /
Entstehung:

Rostock

Rostock: Universität Rostock

2022