| title: |
| Massenspektrometrische Präzisionsanalyse eines Antikörper-Bindemotivs in der Linker-Region
von C2H2-Zinkfinger-Proteinen: von der Charakterisierung der Epitop-Feinstruktur zur
Antikörper-basierten Tumordiagnostik am Beispiel des Endometriumkarzinoms |
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| contributing persons: |
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| contributing corporate bodies: |
| Universität Rostock[Grad-verleihende Institution] |
 |
38329-6 |
| Universitätsmedizin Rostock[Grad-verleihende Institution] |
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1029510660 |
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| abstract: |
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Mittels ITEM-TWO untersuchte ich, inwieweit eine Tumordiagnostik mit dem anti-HpTGEKP-Antikörper
von Aminosäureaustauschen/Phosphorylierungen im bipolaren Antikörperbindemotiv abhängig
ist. Getestet wurden 15 Peptidsequenzen: Die 10 häufigsten C2H2-Zinkfinger-Linker
und der im Endometriumkarzinom im ZNF331-Gen gehäuft vorkommende H11Y-Austausch, inkl.
Phosphorylierungen. Dabei führte die präzisere Differenzierung von Antikörperbindungen
zur Einteilung in ‚orthodoxe/unorthodoxe‘ Epitop-Paratop-Bindungstypen, was wegweisend
zur Charakterisierung künftiger Antikörperdiagnostika/-therapeutika ist.
[German] |
|
I applied ITEM-TWO to investigate whether tumor diagnosis with the anti-HpTGEKP antibody
depended on amino acid exchanges/phosphorylations within its bipolar binding motif.
15 peptides were tested: The 10 most common C2H2 zinc finger linkers and linkers carrying
the H11Y exchange plus phosphorylations, caused by mutation of the ZNF331 gene in
uterine corpus endometrial carcinoma. To reach my goal I needed to further differentiate
specific antibody binding introducing a novel 'orthodox/unorthodox' binding type,
which will improve assessment of future antibody-based diagnostics/therapeutics.
[English] |
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| document type: |
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| institution: |
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| language: |
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| subject class (DDC): |
| 610 Medical sciences Medicine |
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publication /
production: |
Rostock
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Rostock: Universität Rostock
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2023
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| statement of responsibility: |
| vorgelegt von Maximilian Scherf |
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| notes: |
| Artikel in Fachzeitschriften: 08/2022 (Erstautor): Epitope fine mapping by mass spectrometry:
Investigations of immune complexes consisting of monoclonal anti‐HpTGEKP- antibody
and zinc finger protein linker phospho‐hexapeptides Scherf M, Danquah BD, Koy C, Lorenz
P, Steinbeck F, Neamtu A, Thiesen H-J, Glocker MO ChemBioChem. 2022;23(20). 01/2023
(Erstautor): Characterization of Phosphorylation-Dependent Antibody Binding to Cancer-Mutated
Linkers of C2H2 Zinc Finger Proteins by Intact Transition Epitope Mapping-Thermodynamic
Weak-Force Order Analysis Scherf M, Koy C, Röwer C, Neamtu A, Glocker MO. J Am Soc
Mass Spectrom. 2023;34(2):171-81. Konferenzbeiträge: 06/2022 (Erstautor): Mass Spectrometric
Binding Strength Characterization of Paratope - Epitope Interactions with Amino Acid
Residue Resolution: Towards Deciphering Molecular “Recognition Codes” Scherf M, Danquah
BD, Koy C, Lorenz P, Steinbeck, F Neamtu A, Thiesen H-J, Glocker MO. 70th ASMS Conference
on Mass Spectrometry and Allied Topics. Minneapolis, Minnesota, USA. 05/2023 (Co-Autor):
Mass Spectrometric ITEM-TWO Investigations and Bio- Computational Analyses of Phosphorylation-Dependent
Antibody Binding to Cancer-Mutated Linkers of C2H2 Zinc Finger Proteins Koy C, Scherf M,
Röwer C, Neamtu A, Glocker MO. 54th Annual Conference of the German Society for Mass
Spectrometry. Dortmund, Germany. 08/2023 (Co-Autor): Mass Spectrometric ITEM-TWO Investigations
and Bio-Computational Analyses of Phosphorylation-Dependent Antibody Binding to Cancer-Mutated
Linkers of C2H2 Zinc Finger Proteins Koy C, Scherf M, Röwer C, Neamtu A, Glocker MO.
16th German Peptide Symposium. Jena, Germany. |
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| identifiers: |
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| access condition: |
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| license/rights statement: |
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| RosDok id: |
rosdok_disshab_0000003075 |
| created / modified: |
18.11.2023 / 20.11.2023
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| metadata license: |
The metadata of this document was dedicated to the public domain
(CC0 1.0 Universal Public Domain Dedication). |
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