Phosphoproteome analysis of the near-haploid cell line HAP1 reveals phosphorylation events originating from PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK)

Hasan Albony

doi:10.18453/rosdok_id00004478

Hasan Albony

Phosphoproteome analysis of the near-haploid cell line HAP1 reveals phosphorylation events originating from PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK)

Universität Rostock, 2021

https://doi.org/10.18453/rosdok_id00004478

Abstract: PBK is a serine/threonine kinase. During mitosis, PBK phosphorylates the inter-zinc finger linker sequence (TGEKP) of hundreds of C2H2 zinc finger proteins and liberates these transcription factors from the condensing chromatin. This study aimed to investigate PBK-dependent phosphorylation events by analyzing the phosphoproteome of both wild and PBK knockout HAP1 cells. The analysis identified candidates belong to DNA- and RNA binding proteins (ADNP and FUBP1) or dynamic regulators of cell organelles (MARCKS and PGRMC1).

Dissertation Freier Zugang

Titel:

Phosphoproteome analysis of the near-haploid cell line HAP1 reveals phosphorylation events originating from PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK)

Beteiligte Personen:

Hasan Albony[VerfasserIn]
	127448958X
Hans-Jürgen Thiesen[AkademischeR BetreuerIn]
	136714013
	Institut für Immunologie, Universität Rostock
Christopher Gerner[AkademischeR BetreuerIn]
	Fakultät für Chemie, Universität Wien
Hugo Istvan Murua Escobar[AkademischeR BetreuerIn]
	12980455X

Beteiligte Körperschaften:

Universität Rostock[Grad-verleihende Institution]
	38329-6
Universitätsmedizin Rostock[Grad-verleihende Institution]
	1029510660

Zusammenfassung:

PBK is a serine/threonine kinase. During mitosis, PBK phosphorylates the inter-zinc finger linker sequence (TGEKP) of hundreds of C2H2 zinc finger proteins and liberates these transcription factors from the condensing chromatin. This study aimed to investigate PBK-dependent phosphorylation events by analyzing the phosphoproteome of both wild and PBK knockout HAP1 cells. The analysis identified candidates belong to DNA- and RNA binding proteins (ADNP and FUBP1) or dynamic regulators of cell organelles (MARCKS and PGRMC1). [Englisch]

Dokumenttyp:

Dissertation

Einrichtung:

Universitätsmedizin

Sprache:

Englisch

Sachgruppe der DNB:

570 Biowissenschaften, Biologie

Veröffentlichung /
Entstehung:

Rostock: Universität Rostock

2021

Verantwortlichkeitsangabe: