| title: |
| Methods development for site-specific investigation of biomolecular structure and
dynamics with DNP-enhanced solid-state NMR |
|
| contributing persons: |
| Thomas Biedenbänder[VerfasserIn] |
 |
0000-0001-6136-4519 |
 |
1393336787 |
| Björn Corzilius[AkademischeR BetreuerIn] |
|
135774721 |
|
University of Rostock |
| Thomas Vosegaard[AkademischeR BetreuerIn] |
|
Aarhus University |
|
| contributing corporate bodies: |
| Universität Rostock[Grad-verleihende Institution] |
|
38329-6 |
| Universität Rostock. Mathematisch-Naturwissenschaftliche Fakultät[Grad-verleihende Institution] |
|
2147083-2 |
|
| |
| abstract: |
|
This work presents advances in Dynamic Nuclear Polarization (DNP), including matrix-free
sample preparation, the combination of heteronuclear cross-relaxation-induced transfer
(SCREAM-DNP) with dipolar recoupling, and relaxation time measurements under DNP conditions.
These three approaches are a promising tool as they facilitate site-specific investigations
of complex biomolecular systems such as amyloid fibrils, ribonucleoprotein complexes,
and the formulation of active substances.
[English] |
|
Diese Arbeit präsentiert Fortschritte in der dynamischen Kernpolarisation (DNP), darunter
matrixfreie Probenvorbereitung, die Kombination von heteronuklearen Kreuzrelaxation-basierten
Polarisationstransfer (SCREAM-DNP) mit dipolarer Rückkopplung, sowie Relaxationszeitmessungen
unter DNP-Bedingungen. Diese drei Ansätze zeigen vielversprechende Ergebnisse und
ermöglichen die ortsspezifische Untersuchung komplexer biomolekularer Systeme wie
amyloide Fibrillen, Ribonukleoproteinkomplexe und Wirkstoffformulierungen.
[German] |
|
| document type: |
|
| institution: |
| Faculty of Mathematics and Natural Sciences |
|
| language: |
|
| subject class (DDC): |
| 530 Physics |
| 540 Chemistry & allied sciences |
|
| extent: |
|
1 Online-Ressource (XI, 162 Seiten)
|
|
| |
publication /
production: |
|
Rostock: Universität Rostock
|
|
December 2024
(normalised date: 2024) |
|
| statement of responsibility: |
| vorgelegt von Thomas Biedenbänder |
|
| |
| identifiers: |
|
| |
| access condition: |
|
| license/rights statement: |
|
|
| RosDok id: |
rosdok_disshab_0000003477 |
| created / modified: |
18.03.2026 / 18.03.2026
|
| metadata license: |
The metadata of this document was dedicated to the public domain
(CC0 1.0 Universal Public Domain Dedication). |
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