| title: |
| Three delivery methods of CRISPR/Cas9 to induce targeted mutations into centromeric
histone H3 (CENH3) in carrots (Daucus carota L.) |
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| other title: |
| Drei Transformationssysteme von CRISPR/Cas9 zur gezielten Veränderung vom zentromerspezifischen
Histon H3 (CENH3) in Karotten (Daucus carota L.)
[translated]
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| contributing persons: |
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| contributing corporate bodies: |
| Universität Rostock[Grad-verleihende Institution] |
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38329-6 |
| Universität Rostock. Mathematisch-Naturwissenschaftliche Fakultät[Grad-verleihende Institution] |
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2147083-2 |
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| abstract: |
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The centromere-specific histone 3 (CENH3) in carrots (Daucus carota) was mutated using
CRISPR/Cas9. Transformation with Agrobacterium tumefaciens and Agrobacterium rhizogenes
resulted in transgenic lines with secondary mutations and lower levels of CENH3. Transient
transformation of carrot protoplasts with CRISPR/Cas9 ribonucleoprotein complexes
(RNPs) resulted in transgene-free and mutated protoplasts. The transgene-free method
was identified as the preferred method due to the absence of secondary mutations.
[English] |
|
Das Zentromer-spezifische Histon 3 (CENH3) in der Karotte (Daucus carota) wurde mittels
CRISPR/Cas9 mutiert. Die Transformation mit Agrobacterium tumefaciens und Agrobacterium
rhizogenes führte zu transgenen Linien mit sekundären Mutationen und geringere Menge
CENH3. Die transiente Transformation von Karottenprotoplasten mit CRISPR/Cas9-Ribonukleoprotein-Komplexen
(RNPs) führte zu transgen-freien und mutierten Protoplasten. Die transgen-freie Methode
wurde wegen fehlender sekundärer Mutation als bevorzugte Methode identifiziert.
[German] |
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| document type: |
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| institution: |
| Faculty of Mathematics and Natural Sciences |
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| language: |
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| subject class (DDC): |
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| extent: |
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1 Online-Ressource (81 Seiten)
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publication /
production: |
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Rostock: Universität Rostock
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2025
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| statement of responsibility: |
| vorgelegt von Katharina Unkel |
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| identifiers: |
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| access condition: |
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| license/rights statement: |
all rights reserved
This work may only be used under the terms of the German Copyright Law (Urheberrechtsgesetz). |
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| RosDok id: |
rosdok_disshab_0000003518 |
| created / modified: |
27.04.2026 / 27.04.2026
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| metadata license: |
The metadata of this document was dedicated to the public domain
(CC0 1.0 Universal Public Domain Dedication). |
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