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Daniela Wetzel

SspA, ein essentielles kleines säurelösliches Sporenprotein (SASP) für das Keimen der Sporen von Clostridium acetobutylicum

Universität Rostock, 2016

Abstract: Six putative SASPs of C. acetobutylicum were investigated for the resistance and outgrowth of endospores and two germination proteases were examined for specific SASP cleaving activity. C. acetobutylicum spores harbor only one major SASP, which could be identified as SspA and account for 93 % of total spore SASPs. The loss of SspA showed dramatic consequences for the development of the new outgrowing cell, a hitherto unique example among spore forming bacteria. Furthermore, enzymatic function of GPR and for the first time germination protease-like activity of an YyaC could be demonstrated.

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