| title: |
| Electrochemical hybridization assay for DNA and asymmetric PCR products detection
by labeling with osmium tetroxide |
|
| contributing persons: |
| Xiaoqi Ni[VerfasserIn] |
 |
1124704744 |
| Gerd-Uwe Flechsig
, Prof. Dr.[AkademischeR BetreuerIn] |
|
123274303 |
|
University at Albany, SUNY, Dept. of Chemistry |
| Peter Langer
, Prof. Dr.[AkademischeR BetreuerIn] |
|
132314614 |
|
University of Rostock, Dept. of Chemistry |
|
| contributing corporate bodies: |
| Universität Rostock, Mathematisch-Naturwissenschaftliche Fakultät[Grad-verleihende Institution] |
|
2147083-2 |
|
| |
| abstract: |
|
This dissertation describes the hybridization assay on gold electrodes for electrochemical
detection of DNA and polymerase chain reaction (PCR) products by means of the electrochemically
active compound osmium tetroxide bipyridine. Single base mismatches can be discriminated
for their different thermal behavior. We further optimized protective-strand design,
surface-probe density, and hybridization temperature. Besides, we also investigated
to obtain labeled single stranded DNA targets from asymmetric PCR after labeling forward
primer with osmium tetroxide for electrochemical detection.
[English] |
|
| document type: |
|
| institution: |
| Faculty of Mathematics and Natural Sciences |
|
| language: |
|
| subject class (DDC): |
| 540 Chemistry & allied sciences |
|
| |
publication /
production: |
Rostock
|
Rostock: Universität Rostock
|
|
2018
|
|
| |
| identifiers: |
|
| |
| access condition: |
|
| license/rights statement: |
all rights reserved
This work may only be used under the terms of the German Copyright Law (Urheberrechtsgesetz). |
|
|
| RosDok id: |
rosdok_disshab_0000001866 |
| created / modified: |
23.03.2018 / 08.08.2023
|
| metadata license: |
The metadata of this document was dedicated to the public domain
(CC0 1.0 Universal Public Domain Dedication). |
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