Electrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxide

Xiaoqi Ni

doi:10.18453/rosdok_id00002044

Xiaoqi Ni

Electrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxide

Universität Rostock, 2018

https://doi.org/10.18453/rosdok_id00002044

Abstract: This dissertation describes the hybridization assay on gold electrodes for electrochemical detection of DNA and polymerase chain reaction (PCR) products by means of the electrochemically active compound osmium tetroxide bipyridine. Single base mismatches can be discriminated for their different thermal behavior. We further optimized protective-strand design, surface-probe density, and hybridization temperature. Besides, we also investigated to obtain labeled single stranded DNA targets from asymmetric PCR after labeling forward primer with osmium tetroxide for electrochemical detection.

Dissertation Freier Zugang

Titel:

Electrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxide

Beteiligte Personen:

Xiaoqi Ni[VerfasserIn]
	1124704744
Gerd-Uwe Flechsig , Prof. Dr.[AkademischeR BetreuerIn]
	123274303
	University at Albany, SUNY, Dept. of Chemistry
Peter Langer , Prof. Dr.[AkademischeR BetreuerIn]
	132314614
	University of Rostock, Dept. of Chemistry

Beteiligte Körperschaften:

Universität Rostock, Mathematisch-Naturwissenschaftliche Fakultät[Grad-verleihende Institution]
	2147083-2

Zusammenfassung:

This dissertation describes the hybridization assay on gold electrodes for electrochemical detection of DNA and polymerase chain reaction (PCR) products by means of the electrochemically active compound osmium tetroxide bipyridine. Single base mismatches can be discriminated for their different thermal behavior. We further optimized protective-strand design, surface-probe density, and hybridization temperature. Besides, we also investigated to obtain labeled single stranded DNA targets from asymmetric PCR after labeling forward primer with osmium tetroxide for electrochemical detection. [Englisch]

Dokumenttyp:

Dissertation

Einrichtung:

Mathematisch-Naturwissenschaftliche Fakultät

Sprache:

Englisch

Sachgruppe der DNB:

540 Chemie

Veröffentlichung /
Entstehung:

Rostock: Universität Rostock

2018

Identifikatoren: